SitesBLAST

F1CMX0 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Rhodococcus rhodochrous (see paper)
33% identity, 59% coverage: 5:70/112 of query aligns to 27:92/394 of F1CMX0

query
sites
F1CMX0

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C68 (= C46) binding
H70 (= H48) binding
C87 (= C65) binding
H90 (= H68) binding

Sites not aligning to the query:

186 binding
245 binding
305 binding

5bokA Ferredoxin component of 3-nitrotoluene dioxygenase from diaphorobacter sp. Strain ds2
26% identity, 90% coverage: 4:104/112 of query aligns to 2:102/102 of 5bokA

query
sites
5bokA

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binding fe2/s2 (inorganic) cluster: C43 (= C46), H45 (= H48), G46 (≠ K49), A48 (≠ G51), C62 (= C65), L64 (= L67), H65 (= H68), P80 (≠ D83)

4qckA Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (ksha) from m. Tuberculosis in complex with 4-androstene-3,17-dione (see paper)
42% identity, 38% coverage: 28:70/112 of query aligns to 36:78/355 of 4qckA

query
sites
4qckA

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binding fe2/s2 (inorganic) cluster: C54 (= C46), H56 (= H48), M57 (≠ K49), G59 (= G51), C73 (= C65), F75 (≠ L67), H76 (= H68), W78 (= W70)

Sites not aligning to the query:

binding 4-androstene-3-17-dione: 173, 191, 206, 218, 220, 280, 284
binding fe (iii) ion: 168, 173, 284

2zylA Crystal structure of 3-ketosteroid-9-alpha-hydroxylase (ksha) from m. Tuberculosis (see paper)
42% identity, 38% coverage: 28:70/112 of query aligns to 36:78/359 of 2zylA

query
sites
2zylA

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binding fe2/s2 (inorganic) cluster: C54 (= C46), H56 (= H48), M57 (≠ K49), C73 (= C65), F75 (≠ L67), H76 (= H68), W78 (= W70)

Sites not aligning to the query:

binding fe (ii) ion: 168, 173, 289

P71875 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
42% identity, 38% coverage: 28:70/112 of query aligns to 49:91/386 of P71875

query
sites
P71875

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C67 (= C46) binding
H69 (= H48) binding
C86 (= C65) binding
H89 (= H68) binding

Sites not aligning to the query:

181 binding
186 binding
304 binding

Q17938 Cholesterol 7-desaturase; Cholesterol desaturase daf-36; Rieske oxygenase DAF-36/Neverland; DAF-36/NVD; EC 1.14.19.21 from Caenorhabditis elegans (see paper)
32% identity, 53% coverage: 29:87/112 of query aligns to 105:167/428 of Q17938

query
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Q17938

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C122 (= C46) mutation to A: Abrogates cholesterol dehydrogenase activity.

Sites not aligning to the query:

234 D→A: Abrogates cholesterol dehydrogenase activity.

A0R635 Putative Rieske 2Fe-2S iron-sulfur protein MSMEG_6410/MSMEI_6242; EC 1.-.-.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
38% identity, 51% coverage: 32:88/112 of query aligns to 450:514/518 of A0R635

query
sites
A0R635

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Sites not aligning to the query:

375 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

4qddA Crystal structure of 3-ketosteroid-9-alpha-hydroxylase 5 (ksha5) from r. Rhodochrous in complex with 1,4-30q-coa (see paper)
28% identity, 78% coverage: 5:91/112 of query aligns to 18:101/366 of 4qddA

query
sites
4qddA

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binding fe2/s2 (inorganic) cluster: C59 (= C46), H61 (= H48), M62 (≠ K49), G64 (= G51), C78 (= C65), F80 (≠ L67), H81 (= H68), W83 (= W70)

Sites not aligning to the query:

4qdcA Crystal structure of 3-ketosteroid-9-alpha-hydroxylase 5 (ksha5) from r. Rhodochrous in complex with fe2/s2 (inorganic) cluster (see paper)
28% identity, 78% coverage: 5:91/112 of query aligns to 18:101/369 of 4qdcA

query
sites
4qdcA

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binding fe2/s2 (inorganic) cluster: C59 (= C46), H61 (= H48), M62 (≠ K49), G64 (= G51), C78 (= C65), F80 (≠ L67), H81 (= H68), W83 (= W70)

Sites not aligning to the query:

binding 4-androstene-3-17-dione: 168, 178, 196, 198, 231, 233
binding fe (iii) ion: 167, 173, 178, 297

1fqtA Crystal structure of the rieske-type ferredoxin associated with biphenyl dioxygenase (see paper)
30% identity, 96% coverage: 3:109/112 of query aligns to 1:108/109 of 1fqtA

query
sites
1fqtA

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binding fe2/s2 (inorganic) cluster: C43 (= C46), H45 (= H48), G46 (≠ K49), C63 (= C65), H66 (= H68), G68 (≠ W70), P82 (≠ E84)

F1CMY8 3-ketosteroid-9-alpha-monooxygenase, oxygenase component; 3-ketosteroid-9-alpha-hydroxylase, oxygenase component; KSH; Androsta-1,4-diene-3,17-dione 9-alpha-hydroxylase; Rieske-type oxygenase; RO; EC 1.14.15.30 from Rhodococcus rhodochrous (see paper)
28% identity, 78% coverage: 5:91/112 of query aligns to 32:115/390 of F1CMY8

query
sites
F1CMY8

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C73 (= C46) binding
H75 (= H48) binding
C92 (= C65) binding
H95 (= H68) binding

Sites not aligning to the query:

181 binding
187 binding
192 binding
311 binding

6wnbA Structure of the rieske non-heme iron oxygenase sxtt with dideoxysaxitoxin bound (see paper)
29% identity, 60% coverage: 4:70/112 of query aligns to 10:78/320 of 6wnbA

query
sites
6wnbA

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binding fe2/s2 (inorganic) cluster: C54 (= C46), H56 (= H48), R57 (≠ K49), C73 (= C65), Y75 (≠ L67), H76 (= H68), W78 (= W70)

Sites not aligning to the query:

7szhA Structure of the rieske non-heme iron oxygenase sxtt with beta- saxitoxinol bound (see paper)
29% identity, 60% coverage: 4:70/112 of query aligns to 9:77/323 of 7szhA

query
sites
7szhA

N

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binding fe2/s2 (inorganic) cluster: C53 (= C46), H55 (= H48), R56 (≠ K49), C72 (= C65), Y74 (≠ L67), H75 (= H68), W77 (= W70)

Sites not aligning to the query:

binding beta-Saxitoxinol: 157, 163, 202, 222, 224, 226, 235, 251, 269, 272
binding fe (iii) ion: 162, 167, 276

6wn3B Structure of the rieske non-heme iron oxygenase sxtt (see paper)
29% identity, 60% coverage: 4:70/112 of query aligns to 11:79/328 of 6wn3B

query
sites
6wn3B

N

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W

W

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V

G

A

D

N

I

V

N

E

D

D

I

C

P

K

L

P

R

G

G

S

A

I

R

T

C

T

V

A

R

R

T

L

P

L

T

G

G

V

K

K

I

L

A

V

V

L

F

W

R

R

T

S

A

Q

H

E

D

Q

E

N

-

S

-

P

V

I

F

Q

A

V

I

W

E

Q

D

D

H

Y

C
|
C

P

P

H
|
H

K
x
R

G

G

G

V

P

P

L

L

S

S

Q

M

G

G

I

E

V

V

H

A

G

N

T

N

A

T

V

L

T

V

C
|
C

P

P

L
x
Y

H
|
H

N

G

W
|
W

binding fe2/s2 (inorganic) cluster: C55 (= C46), H57 (= H48), R58 (≠ K49), C74 (= C65), Y76 (≠ L67), H77 (= H68), W79 (= W70)

Sites not aligning to the query:

binding fe (iii) ion: 164, 169, 280

Q8GI16 Ferredoxin CarAc; Carbazole 1,9a-dioxygenase, ferredoxin component; CARDO from Pseudomonas resinovorans (see 2 papers)
31% identity, 69% coverage: 1:77/112 of query aligns to 1:77/107 of Q8GI16

query
sites
Q8GI16

M

M

T

N

M

Q

N

I

W

W

I

L

A

K

I

V

G

C

D

A

I

A

N

S

D

D

I

M

-

Q

P

P

L

G

R

T

G

I

A

R

R

R

C

V

V

N

R

R

T

V

P

G

T

A

G

A

K

P

I

L

A

A

V

V

F

Y

R

R

T

V

A

G

H

-

D

D

E

Q

V

F

F

Y

A

A

I

T

E

E

D

D

H

T

C
|
C

P

T

H
|
H

K

G

G

I

G

A

P

S

L

L

S

S

Q

E

G

G

I

T

V

L

H

D

G

G

T

D

A

V

V

I

T

E

C
|
C

P

P

L

F

H
|
H

N

G

W

G

V

A

I

F

S

N

L

V

E

C

T

T

G

G

C46 (= C46) binding
H48 (= H48) binding
C65 (= C65) binding
H68 (= H68) binding

4nbbE Carbazole- and oxygen-bound oxygenase with ile262 replaced by val and ferredoxin complex of carbazole 1,9a-dioxygenase (see paper)
31% identity, 65% coverage: 5:77/112 of query aligns to 4:76/114 of 4nbbE

query
sites
4nbbE

W

W

I

L

A

K

I

V

G

C

D

A

I

A

N

S

D

D

I

M

-

Q

P

P

L

G

R

T

G

I

A

R

R

R

C

V

V

N

R

R

T

V

P

G

T

A

G

A

K

P

I

L

A

A

V

V

F

Y

R

R

T

V

A

G

H

-

D

D

E

Q

V

F

F

Y

A

A

I

T

E

E

D

D

H

T

C
|
C

P

T

H
|
H

K
x
G

G

I

G

A

P

S

L

L

S

S

Q

E

G

G

I

T

V

L

H

D

G

G

T

D

A

V

V

I

T

E

C
|
C

P

P

L
x
F

H
|
H

N

G

W
x
G

V

A

I

F

S

N

L

V

E

C

T

T

G

G

binding fe2/s2 (inorganic) cluster: C45 (= C46), H47 (= H48), G48 (≠ K49), C64 (= C65), F66 (≠ L67), H67 (= H68), G69 (≠ W70)

7szfA Structure of the rieske non-heme iron oxygenase gxta with beta- saxitoxinol bound (see paper)
30% identity, 60% coverage: 4:70/112 of query aligns to 10:78/318 of 7szfA

query
sites
7szfA

N

N

W

W

I

Y

A

V

I

V

G

A

D

K

I

V

N

E

D

D

I

C

P

R

L

P

R

G

G

S

A

I

R

T

C

T

V

A

R

H

T

L

P

L

T

G

G

V

K

K

I

L

A

V

V

L

F

W

R

R

T

-

A

S

H

H

D

E

E

Q

V

N

F

S

A

P

I

I

E

Q

-

V

-

W

-

Q

D

D

H

Y

C
|
C

P

P

H
|
H

K
x
R

G

G

G

V

P

P

L

L

S

S

Q

M

G

G

I

E

V

V

H

A

G

N

T

N

A

T

V

L

T

V

C
|
C

P

P

L
x
Y

H
|
H

N

G

W
|
W

binding fe2/s2 (inorganic) cluster: C54 (= C46), H56 (= H48), R57 (≠ K49), C73 (= C65), Y75 (≠ L67), H76 (= H68), W78 (= W70)

Sites not aligning to the query:

binding beta-Saxitoxinol: 158, 164, 217, 219, 230, 246, 264, 267
binding fe (iii) ion: 163, 168, 271

Query Sequence

>SM_b20985 FitnessBrowser__Smeli:SM_b20985
MTMNWIAIGDINDIPLRGARCVRTPTGKIAVFRTAHDEVFAIEDHCPHKGGPLSQGIVHG
TAVTCPLHNWVISLETGKALGADEGEVRTIPIRNDNGALFVALESLALAAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory