SitesBLAST

P0A6A8 Acyl carrier protein; ACP; Cytosolic-activating factor; CAF; Fatty acid synthase acyl carrier protein from Escherichia coli (strain K12) (see 4 papers)
62% identity, 100% coverage: 1:78/78 of query aligns to 1:78/78 of P0A6A8

query
sites
P0A6A8

M
|
M

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D

D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

M1 (= M1) modified: Initiator methionine, Removed
S37 (= S37) modified: O-(pantetheine 4'-phosphoryl)serine; mutation S->A,T: Loss of phosphopantetheinylation, and inhibition of cell growth.

P80922 Acyl carrier protein; ACP from Oceanospirillum linum (see paper)
62% identity, 94% coverage: 1:73/78 of query aligns to 1:73/77 of P80922

query
sites
P80922

M
|
M

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

V

I

S

D

E

H

Q

L

L

G

G

V

V

D

K

A

E

E

E

K

E

V

I

S

T

E

N

G

A

A

S

S

S

F

F

I

V

D

D

L

L

G

G

A

A

D

D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

E

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

E

A

A

V

I

K

D

F

Y

I

V

M1 (= M1) modified: Initiator methionine, Removed
S37 (= S37) modified: O-(pantetheine 4'-phosphoryl)serine

5vcbD Crystal structure of holo-(acyl-carrier-protein) synthase:holo(acyl- carrier-protein) complex from escherichia coli. (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 5vcbD

query
sites
5vcbD

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D
x
E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding 4'-phosphopantetheine: D35 (= D36), S36 (= S37), E60 (≠ D61)

4kehC Crosslinked crystal structure of type ii fatty synthase dehydratase, faba, and acyl carrier protein, acpp (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 4kehC

query
sites
4kehC

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D

D

S
|
S

L
|
L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

binding N-{3-[dihydroxy(nonyl)-lambda~4~-sulfanyl]propyl}-N~3~-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alaninamide: S36 (= S37), L37 (= L38)

3ny7B Stas domain of ychm bound to acp (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 3ny7B

query
sites
3ny7B

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L
|
L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding 3-{[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-3-oxopropanoic acid: D35 (= D36), S36 (= S37), L37 (= L38)

2faeA Crystal structure of e. Coli decanoyl-acp (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 2faeA

query
sites
2faeA

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F
|
F

I
x
V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F
x
L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D
x
E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding s-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate: F28 (= F29), V29 (≠ I30), S36 (= S37), L46 (≠ F47), E60 (≠ D61)

2fadA Crystal structure of e. Coli heptanoyl-acp (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 2fadA

query
sites
2fadA

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F
|
F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T
|
T

V

V

E

E

L

L

V
|
V

M

M

A

A

F
x
L

E

E

F

F

G

D

V
x
T

E

E

I
|
I

P

P

D

D

D
x
E

A

E

A
|
A

D
x
E

S

K

I
|
I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F
x
Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding s-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) heptanethioate: F28 (= F29), S36 (= S37), T39 (= T40), V43 (= V44), L46 (≠ F47), T52 (≠ V53), I54 (= I55), A59 (= A60), I62 (= I63), Y71 (≠ F72)
binding zinc ion: E57 (≠ D58), E60 (≠ D61)

2facA Crystal structure of e. Coli hexanoyl-acp (see paper)
61% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 2facA

query
sites
2facA

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F
|
F

I
x
V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T
|
T

V

V

E

E

L

L

V
|
V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A
|
A

D

E

S

K

I
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I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding s-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) hexanethioate: F28 (= F29), V29 (≠ I30), S36 (= S37), T39 (= T40), V43 (= V44), A59 (= A60), I62 (= I63)

2ehtA Crystal structure of acyl carrier protein from aquifex aeolicus (form 2)
59% identity, 90% coverage: 4:73/78 of query aligns to 2:71/77 of 2ehtA

query
sites
2ehtA

I

L

A

E

E

E

R

R

V

V

K

K

K
x
E

I

I

V

I

I

A

D
x
E

H

Q

L

L

G

G

V

V

D

E

A

K

E

E

K

K

V

I

S

T

E

P

G

E

A

A

S

K

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S

S

L

L

D

D

T

V

V

V

E

E

L

L

V

I

M

M

A

A

F

F

E

E

F

F

G

G

V

I

E

E

I

I

P

P

D

D

D

E

A

D

A

A

D

E

S

K

I

I

L

Q

T

T

V

V

G

G

D

D

A

V

V

I

K

N

F

Y

I

L

active site: D34 (= D36)
binding zinc ion: E8 (≠ K10), E12 (≠ D14)

1l0iA Crystal structure of butyryl-acp i62m mutant (see paper)
60% identity, 99% coverage: 2:78/78 of query aligns to 1:77/77 of 1l0iA

query
sites
1l0iA

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T
|
T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E
|
E

E

E

F

F

G

D

V

T

E
|
E

I

I

P

P

D

D

D

E

A

E

A

A

D
x
E

S

K

I
x
M

L
x
T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

E

N

K

G

A

H

Q

Q

A

A

active site: D35 (= D36)
binding thiobutyric acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: S36 (= S37), T39 (= T40), E60 (≠ D61), M62 (≠ I63), T63 (≠ L64)
binding zinc ion: E47 (= E48), E53 (= E54)

P80918 Acyl carrier protein; ACP from Comamonas testosteroni (Pseudomonas testosteroni) (see paper)
56% identity, 100% coverage: 1:78/78 of query aligns to 1:78/78 of P80918

query
sites
P80918

M
|
M

S

S

D

D

I

I

A

E

E

A

R

R

V

V

K

K

I

I

V

I

I

A

D

E

H

Q

L

L

G

G

V

V

D

E

A

E

E

S

K

Q

V

V

S

T

E

N

G

E

A

K

S

A

F

F

I

V

D

A

D

D

L

L

G

G

A

A

D

D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

E

D

E

E

F

F

G

G

V

I

E

E

I

I

P

P

D

D

D

E

A

D

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

N

A

A

V

I

K

D

F

Y

I

A

E

N

K

T

A

H

Q

Q

A

A

M1 (= M1) modified: Initiator methionine, Removed
S37 (= S37) modified: O-(pantetheine 4'-phosphoryl)serine

2qnwA Toxoplasma gondii apicoplast-targeted acyl carrier protein
59% identity, 96% coverage: 4:78/78 of query aligns to 6:80/80 of 2qnwA

query
sites
2qnwA

I

L

A

L

E
|
E

R

R

V

V

K

K

K
x
D

I

V

V

V

I

A

D

D

H

Q

L

L

G

G

V

V

D

D

A

R

E

A

K

R

V

I

S

N

E

P

G

E

A

S

S

N

F

F

I

I

D

K

D

D

L

L

G

D

A

A

D
|
D

S

S

L

L

D

D

T

S

V

V

E

E

L

L

V

V

M

M

A

A

F

F

E

E

E

K

F

F

G

G

V

V

E

S

I

I

P

P

D

D

D

E

A

E

A

A

D

S

S

K

I

I

L

A

T

T

V

V

G

Q

D

D

A

A

V

L

K

S

F

Y

I

I

E
|
E

K

K

A

A

Q

K

A

S

active site: D38 (= D36)
binding zinc ion: E8 (= E6), D12 (≠ K10), E76 (= E74)

Sites not aligning to the query:

binding zinc ion: 3

6n3pI Crosslinked acpp=fabz complex from e. Coli type ii fas (see paper)
63% identity, 92% coverage: 2:73/78 of query aligns to 2:73/76 of 6n3pI

query
sites
6n3pI

S

S

D

T

I

I

A

E

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

active site: D36 (= D36)
binding N~3~-{(2R)-4-[(dihydroxyphosphanyl)oxy]-2-hydroxy-3,3-dimethylbutanoyl}-N-(3-{[(1Z)-pent-1-en-1-yl]sulfonyl}propyl)-beta-alaninamide: D36 (= D36), S37 (= S37)

5usrK Crystal structure of human nfs1-isd11 in complex with e. Coli acyl- carrier protein at 3.09 angstroms (see paper)
63% identity, 87% coverage: 6:73/78 of query aligns to 2:69/69 of 5usrK

query
sites
5usrK

E

E

R

R

V

V

K

K

I

I

V

I

I

G

D

E

H

Q

L

L

G

G

V

V

D

K

A

Q

E

E

K

E

V

V

S

T

E

N

G

N

A

A

S

S

F

F

I

V

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T

T

V

V

E

E

L

L

V

V

M

M

A

A

F

L

E

E

F

F

G

D

V

T

E

E

I

I

P

P

D

D

D

E

A

E

A

A

D

E

S

K

I

I

L

T

T

T

V

V

G

Q

D

A

A

A

V

I

K

D

F

Y

I

I

active site: D32 (= D36)
binding S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate: D32 (= D36), S33 (= S37)

P80643 Acyl carrier protein; ACP from Bacillus subtilis (strain 168) (see paper)
58% identity, 99% coverage: 1:77/78 of query aligns to 1:77/77 of P80643

query
sites
P80643

M

M

S

A

D

D

I

T

A

L

E

E

R

R

V

V

K

T

K

K

I

I

V

I

I

V

D

D

H

R

L

L

G

G

V

V

D

D

A

E

E

A

K

D

V

V

S

K

E

L

G

E

A

A

S

S

F

F

I

K

D

E

D

D

L

L

G

G

A

A

D

D

S
|
S

L

L

D

D

T

V

V

V

E

E

L

L

V

V

M

M

A

E

F

L

E

E

E

D

E

E

F

F

G

D

V

M

E

E

I

I

P

S

D

D

D

E

A

D

A

A

D

E

S

K

I

I

L

A

T

T

V

V

G

G

D

D

A

A

V

V

K

N

F

Y

I

I

E

Q

K

N

A

Q

Q

Q

S37 (= S37) modified: O-(pantetheine 4'-phosphoryl)serine

2x2bA Crystal structure of malonyl-acp (acyl carrier protein) from bacillus subtilis (see paper)
59% identity, 96% coverage: 1:75/78 of query aligns to 1:75/76 of 2x2bA

query
sites
2x2bA

M

M

S

A

D

D

I

T

A

L

E

E

R

R

V

V

K

T

K

K

I

I

V

I

I

V

D

D

H

R

L

L

G

G

V

V

D

D

A

E

E

A

K

D

V

V

S

K

E

L

G

E

A

A

S

S

F

F

I
x
K

D

E

D

D

L

L

G

G

A

A

D
|
D

S
|
S

L

L

D

D

T

V

V

V

E

E

L

L

V

V

M

M

A

E

F

L

E

E

E

D

E

E

F

F

G

D

V

M

E

E

I

I

P

S

D

D

D

E

A

D

A

A

D

E

S

K

I

I

L

A

T

T

V

V

G

G

D

D

A

A

V

V

K

N

F

Y

I

I

E

Q

K

N

active site: D36 (= D36)
binding 3-{[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]sulfanyl}-3-oxopropanoic acid: K30 (≠ I30), S37 (= S37)

Query Sequence

>SMc00573
MSDIAERVKKIVIDHLGVDAEKVSEGASFIDDLGADSLDTVELVMAFEEEFGVEIPDDAA
DSILTVGDAVKFIEKAQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory