SitesBLAST

E190 (= E272) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A274) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G278) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P291) mutation to A: No effect.

P02916 Maltose/maltodextrin transport system permease protein MalF from Escherichia coli (strain K12) (see 4 papers)
23% identity, 62% coverage: 93:326/380 of query aligns to 204:455/514 of P02916

query
sites
P02916

F

F

S

S

S

G

R

T

E

Q

P

P

T

L

K

Y

F

T

K

L

P

D

G

G

E

D

T

G

A

T

E

L

L

T

N

N

D

N

G

Q

E

S

R

G

L

V

T

K

V

Y

Q

R

S

P

D

N

G

N

S

Q

F

I

E

G

I

F

V

Y

S

Q

D

S

Q

I

R

T

M

A

E

D

G

G

S

N

R

W

G

G

Q

D

R

E

I

K

F

L

-

S

-

P

-

G

F

Y

T

T

A

V

T

T

P

G

P

W

R

K

F

-

T

-

L

-

D

-

N

N

Y

F

A

T

E

R

V

V

L

F

S

T

A

D

A

E

G

G

I

I

G

Q

R

K

S

P

F

F

L

L

N

A

S

I

L

F

T

V

V

W

A

T

V

V

P

V

S

F

T

S

V

L

I

I

P

T

I

V

L

F

I

L

A

T

A

V

F

A

A

V

A

G

Y

M

A

V

L

L

A

A

-

C

-

L

-

V

-

Q

W

W

M

E

P

A

F

L

P

R

G

G

R

K

A

A

V

V

L

Y

L

R

A

V

V

L

V

I

G

L

L

P

L

Y

V

A

V

V

P

P

L

S

Q

F

M

I

S

S

L

I

I
x
L

P

I

L

F

L

K

Q

G

L

L

Y

F

N

N

G

Q

-

S

-

F

-

G

-

E

-

I

-

N

-

M

V

L

G

S

A

A

F

L

F

F

G

G

V

V

-

K

-

P

-

A

-

W

-

F

-

S

-

D

-

P

-

T

S

T

A

A

K

R

T

T

Y

M

M
x
L

G

I

I

I

W

-

L

-

A

V

H
x
N

T

T

G

W

F

L

G
|
G

L

Y

P

P

L

Y

A

M

I

M

Y

I

L

L

L

C

R

M

N

G

Y

L

M

L

A

K

G

A

L

I

P

P

R

D

E

D

I

L

M

Y

E
|
E

S

A

A
x
S

R

A

V

M

D

D

G
|
G

A

A

S

G

D

P

F

F

D

Q

I

N

F

F

V

F

K

K

I

I

I

T

L

L

P
|
P

L

L

S

L

F

I

P

K

A

P

L

L

A

T

S

P

F

L

A

M

I

I

F

A

Q

S

F

F

L

A

W

F

T

N

W

F

N

N

D

N

L

F

L

V

V

L

A

I

I

Q

V

L

F

L

L

T

G

N

A

G

G

G

D

P

D

D

K

R

L

L

L334 (≠ I220) mutation to W: Ability to transport lactose in a saturable manner.
L372 (≠ M241) mutation to W: Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose.
N376 (≠ H247) mutation N->K,H: No growth on maltose.
G380 (= G251) mutation G->C,S: No growth on maltose.
E401 (= E272) mutation E->A,C,K,L: Reduction of transport rate.
S403 (≠ A274) mutation S->C,D,K,L: Reduction of transport rate.
G407 (= G278) mutation G->A,P: No effect.
P420 (= P291) mutation to A: No effect.

4ki0F Crystal structure of the maltose-binding protein/maltose transporter complex in an outward-facing conformation bound to maltohexaose (see paper)
25% identity, 46% coverage: 153:326/380 of query aligns to 248:440/490 of 4ki0F

query
sites
4ki0F

N

N

Y

F

A

T

E

R

V

V

L

F

S

T

A

D

A

E

G

G

I

I

G

Q

R

K

S

P

F

F

L

L

N

A

S

I

L

F

T

V

V

W

A

T

V

V

P

V

S

F

T

S

V

L

I

I

P

T

I

V

L

F

I

L

A

T

A

V

F

A

A

V

A

G

Y

M

A

V

L

L

A

A

-

C

-

L

-

V

-

Q

W

W

M

E

P

A

F

L

P

R

G

G

R

K

A

A

V

V

L

Y

L

R

A

V

V

L

V

I

G

L

L

P

L
x
Y

V

A

V

V

P

P

L

S

Q

F

M

I

S

S

L

I

I

L

P

I

L

F

L

K

Q

G

L

L

Y

F

N

N

G

Q

-

S

-

F

-

G

-

E

-

I

-

N

-

M

V

L

G

S

A

A

F

L

F

F

G

G

V

V

-

K

-

P

-

A

-

W

-

F

-

S

-

D

-

P

-

T

S

T

A

A

K

R

T

T

Y

M

M

L

G

I

I

I

W

-

L

-

A

V

H
x
N

T

T

G

W

F

L

G
|
G

L

Y

P

P

L
x
Y

A

M

I

M

Y

I

L

L

L

C

R

M

N

G

Y

L

M

L

A

K

G

A

L

I

P

P

R

D

E

D

I

L

M

Y

E

E

S

A

A

S

R

A

V

M

D

D

G

G

A

A

S

G

D

P

F

F

D

Q

I

N

F

F

V

F

K

K

I

I

I

T

L

L

P

P

L

L

S

L

F

I

P

K

A

P

L

L

A

T

S

P

F

L

A

M

I

I

F

A

Q
x
S

F

F

L

A

W

F

T
x
N

W

F

N

N

D

N

L

F

L

V

V

L

A

I

I

Q

V

L

F

L

L

T

G

N

A

G

G

G

D

P

D

D

K

R

L

L

binding alpha-D-glucopyranose: Y310 (≠ L211), N361 (≠ H247), G365 (= G251), Y368 (≠ L254), S418 (≠ Q304), N422 (≠ T308)

Query Sequence

>SMc03063 FitnessBrowser__Smeli:SMc03063
MNPSRRSPLTWAVHLSVLLLVLLWTLPTAGLLISSLRDKDQLAVSGWWTALSSSSRNAVV
RAPSAEDQVERDGKFVISGNLLEGRGEVSAFGFSSREPTKFKPGETAELNDGERLTVQSD
GSFEIVSDQRMEGSRGQRIFFTATTPPRFTLDNYAEVLSAAGIGRSFLNSLTVAVPSTVI
PILIAAFAAYALAWMPFPGRAVLLAVVVGLLVVPLQMSLIPLLQLYNGVGAFFGVSAKTY
MGIWLAHTGFGLPLAIYLLRNYMAGLPREIMESARVDGASDFDIFVKIILPLSFPALASF
AIFQFLWTWNDLLVAIVFLGAGDDKLVLTGRLVNLLGSRGGNWEILTASAFITIVVPLIV
FFALQRYLVRGLLAGSVKGG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory