SitesBLAST

P0A890 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli (strain K12) (see 2 papers)
32% identity, 94% coverage: 6:77/77 of query aligns to 8:79/81 of P0A890

query
sites
P0A890

P

P

D

D

Y

H

Q

T

L

L

D

D

M

A

V

L

G

G

E

L

P

R

C
|
C

P

P

Y
x
E

P

P

A

V

V

M

A

M

T

V

L

R

E

K

A

T

M

V

P

R

T

N

L

M

K

Q

P

P

G

G

E

E

I

T

L

L

E

L

V

I

I

I

S

A

D

D

C

D

P

P

Q

A

S

T

I

T

N

R

N

D

I

I

P

P

L

G

D

F

A
x
C

K

T

N

F

H

M

G

E

Y

H

K

E

V

L

L

V

E

A

I

K

E

E

Q

T

N

D

G

G

P

L

T

P

I

Y

R

R

Y

Y

L

L

I

I

Q

R

R

K

C19 (= C17) active site, Cysteine persulfide intermediate; mutation to S: Unable to restore mnm5s2U formation in a tusA-deficient mutant. Unable to accept sulfur from IscS.
E21 (≠ Y19) mutation to K: In sirA1; affects the stability of sigma-S during the logarithmic growth phase.
C56 (≠ A54) mutation to S: Able to restore mnm5s2U formation in a tusA-deficient mutant.

P0A892 Sulfur carrier protein TusA; Sulfur mediator TusA; Sulfur transfer protein TusA; tRNA 2-thiouridine synthesizing protein A from Escherichia coli O157:H7 (see paper)
32% identity, 94% coverage: 6:77/77 of query aligns to 8:79/81 of P0A892

query
sites
P0A892

P

P

D

D

Y

H

Q

T

L

L

D

D

M

A

V

L

G

G

E

L

P

R

C

C

P

P

Y
x
E

P

P

A

V

V
x
M

A

M

T

V

L
x
R

E
x
K

A
x
T

M
x
V

P
x
R

T
x
N

L
x
M

K
x
Q

P
|
P

G

G

E

E

I

T

L

L

E

L

V

I

I

I

S

A

D

D

C
x
D

P

P

Q

A

S

T

I

T

N
x
R

N
x
D

I

I

P

P

L

G

D

F

A

C

K

T

N
x
F

H

M

G

E

Y

H

K

E

V

L

L

V

E

A

I

K

E

E

Q

T

N

D

G

G

P

L

T

P

I

Y

R

R

Y

Y

L

L

I

I

Q

R

R

K

E21 (≠ Y19) mutation to A: Decreased binding of IscS.
M24 (≠ V22) mutation to R: No binding of IscS, 50% modified tRNA produced.
R27 (≠ L25) mutation R->D,E: No binding of IscS, 19% modified tRNA produced (for R27E).
27:35 (vs. 25:33, 11% identical) mutation to DKTVRNMQS: No modified tRNA produced.
R31 (≠ P29) mutation to A: No binding of IscS, 30% modified tRNA produced.
D45 (≠ C43) mutation to A: Binds IscS, 56% modified tRNA produced.
R50 (≠ N48) mutation to A: Binds IscS, 67% modified tRNA produced.
D51 (≠ N49) mutation to A: Binds IscS, 67% modified tRNA produced.
F58 (≠ N56) mutation to A: No binding of IscS, 67% modified tRNA produced.

D3RPC0 Sulfur carrier protein TusA from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum) (see paper)
28% identity, 92% coverage: 7:77/77 of query aligns to 5:75/76 of D3RPC0

query
sites
D3RPC0

D

D

Y

Q

Q

E

L

L

D

D

M

A

V

S

G

G

E

L

P

N

C
|
C

P

P

Y

L

P

P

A

I

V

L

A

R

T

A

L

K

E

K

A

T

M

L

P

N

T

A

L

M

K

S

P

S

G

G

E

Q

I

V

L

L

E

H

V

V

I

I

S

A

D

T

C

D

P

P

Q

G

S

S

I

V

N

K

N

D

I

F

P

D

L

A

D

F

A

A

K

K

N

Q

H

T

G

G

Y

N

K

E

V

L

L

M

E

E

I

S

E

K

Q

E

N

E

G

G

P

G

T

K

I

F

R

H

Y

F

L

L

I

I

Q

K

R

K

C15 (= C17) active site, Cysteine persulfide intermediate; mutation to S: Loss of sulfur binding in the presence of sulfide. Loss of interaction with DsrEFH proteins.

Query Sequence

>SO0109
MSQYIPDYQLDMVGEPCPYPAVATLEAMPTLKPGEILEVISDCPQSINNIPLDAKNHGYK
VLEIEQNGPTIRYLIQR

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory