SitesBLAST

P00208 Ferredoxin; 2[4Fe-4S] ferredoxin from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum) (see 3 papers)
65% identity, 96% coverage: 1:82/85 of query aligns to 1:82/83 of P00208

query
sites
P00208

M
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M

A

A

L

L

L

M

I

I

D

T

D

D

S

E

C
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C

I

I

N

N

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C

D

D

M
x
V

C
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C

E

E

P

P

E

E

C
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C

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P

N

N

Q

G

A

A

I

I

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M

Q

G

G

E

D

E

E

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Y

Y

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L

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M1 (= M1) modified: Initiator methionine, Removed
C9 (= C9) binding [4Fe-4S] cluster
C12 (= C12) binding [4Fe-4S] cluster
V14 (≠ M14) mutation to G: Increases the reduction potential of cluster 1 by approximately 50 mV.
C15 (= C15) binding [4Fe-4S] cluster
C19 (= C19) binding [4Fe-4S] cluster
C38 (= C38) binding [4Fe-4S] cluster
C41 (= C41) binding [4Fe-4S] cluster
C50 (= C50) binding [4Fe-4S] cluster
C54 (= C54) binding [4Fe-4S] cluster
C58 (= C58) mutation to A: Increases the reduction potential of cluster 1 by approximately 50 mV.; mutation to S: No change in the reduction potential values of the clusters.

3eunA Crystal structure of the 2[4fe-4s] c57a ferredoxin variant from allochromatium vinosum (see paper)
63% identity, 95% coverage: 2:82/85 of query aligns to 1:81/81 of 3eunA

query
sites
3eunA

A

A

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I

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D

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C

I
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I

N

N

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C

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D

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C

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E

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C

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A

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binding iron/sulfur cluster: I4 (= I5), C8 (= C9), I9 (= I10), C11 (= C12), D12 (= D13), C14 (= C15), C18 (= C19), A22 (= A23), I23 (= I24), Y30 (= Y31), I32 (= I33), C37 (= C38), T38 (= T39), E39 (= E40), C40 (= C41), S47 (≠ P48), C49 (= C50), C53 (= C54), P54 (= P55), I58 (= I59)

P52102 Ferredoxin YfhL; EcFd from Escherichia coli (strain K12) (see paper)
64% identity, 93% coverage: 1:79/85 of query aligns to 1:80/86 of P52102

query
sites
P52102

M

M

A

A

L

L

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D

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N

N

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D

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C

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C

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C9 (= C9) binding [4Fe-4S] cluster
C12 (= C12) binding [4Fe-4S] cluster
C15 (= C15) binding [4Fe-4S] cluster
C19 (= C19) binding [4Fe-4S] cluster
C38 (= C38) binding [4Fe-4S] cluster
C41 (= C41) binding [4Fe-4S] cluster
C50 (= C50) binding [4Fe-4S] cluster
C54 (= C54) binding [4Fe-4S] cluster

2zvsA Crystal structure of the 2[4fe-4s] ferredoxin from escherichia coli (see paper)
63% identity, 92% coverage: 2:79/85 of query aligns to 1:79/81 of 2zvsA

query
sites
2zvsA

A

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L

L

L

I

I

D

T

D

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S

K

C
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C

I
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I

N

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C
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C

D

D

M

M

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C

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A

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T

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E

C
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C

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V

G

G

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H

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binding iron/sulfur cluster: L2 (= L3), C8 (= C9), I9 (= I10), C11 (= C12), C14 (= C15), C18 (= C19), Y30 (= Y31), I32 (= I33), C37 (= C38), T38 (= T39), E39 (= E40), C40 (= C41), C49 (= C50), C53 (= C54), P54 (= P55)

1rgvA Crystal structure of the ferredoxin from thauera aromatica (see paper)
53% identity, 94% coverage: 2:81/85 of query aligns to 1:80/80 of 1rgvA

query
sites
1rgvA

A

A

L

L

L

Y

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D

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x
T

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x
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D

M

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C

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E

E

C
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C

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E

L

L

L

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K

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G

G

binding iron/sulfur cluster: C8 (= C9), T9 (≠ I10), A10 (≠ N11), C11 (= C12), D12 (= D13), C14 (= C15), C18 (= C19), P19 (= P20), Y30 (= Y31), C37 (= C38), E39 (= E40), C40 (= C41), C49 (= C50), C53 (= C54), C57 (= C58)

P00198 4Fe-4S ferredoxin FdxA; Ferredoxin from Gottschalkia acidurici (strain ATCC 7906 / DSM 604 / BCRC 14475 / CIP 104303 / KCTC 5404 / NCIMB 10678 / 9a) (Clostridium acidurici) (see 3 papers)
44% identity, 67% coverage: 1:57/85 of query aligns to 1:51/56 of P00198

query
sites
P00198

M
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M

A
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A

L
x
Y

L
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V

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I

D
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N

D
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C

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I

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G

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C

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E

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G

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Y

-

D

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K

-

P
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G

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G

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V

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C

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P

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D
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D

M1 (= M1) modified: Initiator methionine, Removed
C9 (= C9) binding [4Fe-4S] cluster
C12 (= C12) binding [4Fe-4S] cluster
C15 (= C15) binding [4Fe-4S] cluster
C19 (= C19) binding [4Fe-4S] cluster
C38 (= C38) binding [4Fe-4S] cluster
C41 (= C41) binding [4Fe-4S] cluster
C44 (= C50) binding [4Fe-4S] cluster
C48 (= C54) binding [4Fe-4S] cluster

Sites not aligning to the query:

2:56 modified: mature protein, 4Fe-4S ferredoxin FdxA

1fcaA Structure of the ferredoxin from clostridium acidurici: model at 1.8 angstroms resolution (see paper)
43% identity, 66% coverage: 2:57/85 of query aligns to 1:50/55 of 1fcaA

query
sites
1fcaA

A

A

L
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Y

L

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I

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N

D

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S

A

C
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C

I
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I

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G

M

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C
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C

E

E

P

P

E

E

C
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C

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P

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A

A

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I

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M

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G

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I
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I

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D

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A

N

D

L

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C

T
x
I

E
x
D

C
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C

V

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G

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H

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D

binding iron/sulfur cluster: Y2 (≠ L3), I4 (= I5), C8 (= C9), I9 (= I10), S10 (≠ N11), C11 (= C12), G12 (≠ D13), C14 (= C15), C18 (= C19), I23 (= I24), Y30 (= Y31), I32 (= I33), C37 (= C38), I38 (≠ T39), D39 (≠ E40), C40 (= C41), G41 (≠ P48), C43 (= C50), C47 (= C54)

Sites not aligning to the query:

binding iron/sulfur cluster: 52

P00195 Ferredoxin from Clostridium pasteurianum (see 2 papers)
41% identity, 66% coverage: 1:56/85 of query aligns to 1:50/56 of P00195

query
sites
P00195

M
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M

A

A

L

Y

L

K

I

I

D

A

D

D

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S

C
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C

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C

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C

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A

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A

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V

M1 (= M1) modified: Initiator methionine, Removed
C9 (= C9) binding [4Fe-4S] cluster

1clfA Clostridium pasteurianum ferredoxin (see paper)
40% identity, 65% coverage: 2:56/85 of query aligns to 1:49/55 of 1clfA

query
sites
1clfA

A

A

L

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I
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I

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A

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D

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S

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N

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C

D
x
G

M
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C

E

A

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E

E

C
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C

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P

N
x
V

Q

N

A

A

I

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M

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G

G

E

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E

S

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I

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F

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I

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N

D

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H

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Y

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D

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K

-

P

G

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x
N

C
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C

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A

S

N

V

V

C
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C

P

P

I

V

binding iron/sulfur cluster: I4 (= I5), C8 (= C9), V9 (≠ I10), C11 (= C12), G12 (≠ D13), A13 (≠ M14), C14 (= C15), C18 (= C19), P19 (= P20), V20 (≠ N21), F30 (≠ Y31), C37 (= C38), I38 (≠ T39), C40 (= C41), N42 (≠ T49), C43 (= C50), C47 (= C54)

Sites not aligning to the query:

binding iron/sulfur cluster: 51

8zqdA Anaerobically isolated active [fefe]-hydrogenase cba5h
42% identity, 67% coverage: 3:59/85 of query aligns to 202:253/626 of 8zqdA

query
sites
8zqdA

L

L

L

T

I
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I

D

T

D

K

S

K

C
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C

I
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I

N
x
G

C
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C

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x
G

M

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C
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C

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G

G

E

E

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L

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K

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K

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E

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I

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D

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Y

N

N

L

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C
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C

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H

C
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G

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H

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x
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C

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S

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C

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C

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I
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I

binding iron/sulfur cluster: I204 (= I5), C208 (= C9), I209 (= I10), G210 (≠ N11), C211 (= C12), G212 (≠ D13), C214 (= C15), C218 (= C19), P219 (= P20), C222 (≠ A23), I223 (= I24), C238 (= C38), T239 (= T39), H240 (≠ E40), C241 (= C41), G242 (≠ P48), C244 (= C50), C248 (= C54), P249 (= P55), I253 (= I59)

Sites not aligning to the query:

binding dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethanethiolatato-1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+): 278, 279, 280, 316, 353, 377, 378, 379, 408, 412, 471, 551, 557
binding iron/sulfur cluster: 354, 409, 551, 552, 553, 557
binding zinc ion: 99, 185, 191, 196

1durA Replacement for 1fdx 2(4fe4s) ferredoxin from (now) peptostreptococcus asaccharolyticus
43% identity, 72% coverage: 2:62/85 of query aligns to 1:54/55 of 1durA

query
sites
1durA

A

A

L
x
Y

L

V

I
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I

D

N

D

D

S

S

C
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C

I
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I

N
x
A

C
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C

D
x
G

M

A

C
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C

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K

P

P

E

E

C
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C

P

P

N

V

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N

A
x
C

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I

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E

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G

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E

S

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Y

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I

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N

D

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C

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x
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E

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C
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C

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C

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N

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P

D

E

binding iron/sulfur cluster: Y2 (≠ L3), I4 (= I5), C8 (= C9), I9 (= I10), A10 (≠ N11), C11 (= C12), G12 (≠ D13), C14 (= C15), C18 (= C19), C22 (≠ A23), I23 (= I24), Y29 (= Y31), I31 (= I33), C36 (= C38), I37 (≠ T39), C39 (= C41), G40 (≠ P48), C42 (= C50), C46 (= C54), P47 (= P55)

Query Sequence

>Shew_2785
MALLIDDSCINCDMCEPECPNQAITMGEEIYEIDPNLCTECVGHYDKPTCVSVCPIDCID
PDPAHVESQDELLVKYHLITGKPID

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory