SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_1973 FitnessBrowser__SynE:Synpcc7942_1973 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P74285 UTP--glucose-1-phosphate uridylyltransferase; Cyanobacterial UDP-glucose pyrophosphorylase; UDP-glucose pyrophosphorylase; UDP-Glc PPase; EC 2.7.7.9 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
79% identity, 94% coverage: 1:367/389 of query aligns to 1:367/388 of P74285

query
sites
P74285

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A8 (= A8) mutation to G: Two-fold decrease in affinity for UTP. No effect on affinity for Glc-1P and on catalytic activity rate.

7whsA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gtp (see paper)
30% identity, 97% coverage: 1:377/389 of query aligns to 1:359/366 of 7whsA

query
sites
7whsA

M

M

K

R

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R

binding guanosine-5'-triphosphate: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G9 (= G9), G11 (= G11), R13 (= R13), A52 (≠ N52), G85 (= G93), N108 (≠ C116), D110 (= D118)
binding magnesium ion: D110 (= D118), D218 (= D231)

7d73E Cryo-em structure of gmppa/gmppb complex bound to gtp (state i) (see paper)
31% identity, 82% coverage: 1:319/389 of query aligns to 1:284/360 of 7d73E

query
sites
7d73E

M

M

K

K

A

A

M

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x
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G
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G

G

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D

S

G

V

V

binding guanosine-5'-triphosphate: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G9 (= G9), G11 (= G11), T12 (= T12), R13 (= R13), A52 (≠ N52), S54 (= S54), E80 (= E79), P83 (≠ A91), G85 (= G93), N108 (≠ C116)

7d72K Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
31% identity, 82% coverage: 1:319/389 of query aligns to 1:284/360 of 7d72K

query
sites
7d72K

M

M

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K

A

A

M

L

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I

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A
x
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A
x
G

G

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G
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x
T

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Y

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D

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G

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E

E

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T

N

T

K

I

I

N
|
N

T

A

G

G

I

M

Y

Y

I

I

F

L

E

S

P

P

E

A

I

V

L

L

N

Q

Y

R

I

I

P

-

S

Q

G

L

Q

Q

E

P

Y

T

D

S

I

I

G
x
E

G

K

D

E

L

V

F

F

P

P

K

I

L

M

V

A

E

K

T

E

G

G

A

Q

P

-

F

L

Y

Y

G

A

I

M

S

E

M

L

D

Q

F

G

Q

F

W

W

I

M

D
|
D

I

I

G

G

K

Q

V

P

P

K

D

D

Y

F

W

L

Q

T

A

G

I

M

R

C

S

L

V

F

L

L

N

Q

G

S

E

L

V

-

R

-

N

-

V

-

A

-

I

-

P

-

G

-

K

R

Q

Q

V

K

R

Q

P

P

G

-

V

-

Y

-

T

-

G

-

L

-

N

-

V

-

A

-

I

-

D

-

W

-

D

-

S

E

V

R

A

L

I

C

E

S

G

G

P

P

V

G

Y

I

I

V

G

G

G

N

M

V

T

L

R

-

I

V

D

D

E

P

G

S

V

A

R

R

I

I

V

G

G

Q

P

N

A

C

M

S

I

I

G

G

P

P

N

N

C

V

H

S

I

L

C

G

K

P

G

G

A

V

T

V

I

V

E

E

N

D

S

G

V

V

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G8 (≠ A8), G85 (= G93), T86 (≠ S94), N108 (≠ C116), S109 (≠ G117), D110 (= D118), N172 (= N183), E194 (≠ G206), D218 (= D231)

7d72E Cryo-em structures of human gmppa/gmppb complex bound to gdp-mannose (see paper)
31% identity, 82% coverage: 1:319/389 of query aligns to 1:284/360 of 7d72E

query
sites
7d72E

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A
x
G

G

G

K

Y

G

G

T

T

R

R

V

L

R

R

P

P

I

L

T

T

Y

L

T

S

I

T

P

P

K

K

P

P

M

L

I

V

P

D

I

F

L

C

Q

N

K

K

P

P

V

I

M

L

E

L

F

H

L

Q

L

V

E

E

L

A

L

L

K

A

E

A

H

A

G

G

F

V

K

D

E

H

V

V

M

I

V

L

N

A

V

V

S
|
S

H

Y

L

M

A

S

Q

Q

E

V

I

L

E

E

S

K

Y

E

F

M

R

K

-

A

D

Q

G

E

Q

Q

R

R

F

L

G

G

V

I

E

R

V

I

A

S

Y

M

S

S

F

H

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

E

E

E

A

P

L

L

G
|
G

S

T

A

A

G

G

G

P

M

L

K

A

R

L

I

A

Q

R

D

D

F

L

S

L

P

S

F

E

F

T

D

A

D

D

T

P

F

F

V

F

V

V

L

L

C

N

G
x
S

D
|
D

A

V

L

I

I

C

D

D

L

F

D

P

L

F

T

Q

E

A

A

M

V

V

R

Q

W

F

H

H

K

R

E

H

K

H

G

G

A

Q

L

E

A

G

T

S

I

I

V

L

M

V

K

T

T

K

V

V

P

-

R

-

E

E

V

P

S

S

S

K

Y

Y

G

G

V

V

T

C

D

E

S

A

D

D

-

T

G

G

R

R

I

I

Q

H

A

R

F

F

Q

V

E

E

K

K

P

P

S

Q

V

V

E

-

A

F

L

V

S

S

T

N

T

K

I

I

N
|
N

T

A

G

G

I

M

Y

Y

I

I

F

L

E

S

P

P

E

A

I

V

L

L

N

Q

Y

R

I

I

P

-

S

Q

G

L

Q

Q

E

P

Y

T

D

S

I

I

G
x
E

G

K

D

E

L

V

F

F

P

P

K

I

L

M

V

A

E

K

T

E

G

G

A

Q

P

-

F

L

Y

Y

G

A

I

M

S

E

M

L

D

Q

F

G

Q

F

W

W

I

M

D
|
D

I

I

G

G

K

Q

V

P

P

K

D

D

Y

F

W

L

Q

T

A

G

I

M

R

C

S

L

V

F

L

L

N

Q

G

S

E

L

V

-

R

-

N

-

V

-

A

-

I

-

P

-

G

-

K

R

Q

Q

V

K

R

Q

P

P

G

-

V

-

Y

-

T

-

G

-

L

-

N

-

V

-

A

-

I

-

D

-

W

-

D

-

S

E

V

R

A

L

I

C

E

S

G

G

P

P

V

G

Y

I

I

V

G

G

G

N

M

V

T

L

R

-

I

V

D

D

E

P

G

S

V

A

R

R

I

I

V

G

G

Q

P

N

A

C

M

S

I

I

G

G

P

P

N

N

C

V

H

S

I

L

C

G

K

P

G

G

A

V

T

V

I

V

E

E

N

D

S

G

V

V

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G8 (≠ A8), S54 (= S54), G85 (= G93), S109 (≠ G117), N172 (= N183), E194 (≠ G206), D218 (= D231)
binding magnesium ion: D110 (= D118), D218 (= D231)

7whtA Cryo-em structure of leishmanial gdp-mannose pyrophosphorylase in complex with gdp-mannose (see paper)
29% identity, 97% coverage: 1:377/389 of query aligns to 1:353/360 of 7whtA

query
sites
7whtA

M

M

K

R

A

A

M

V

I

I

L
|
L

A
x
V

A

G

G
|
G

K

F

G
|
G

T

T

R

R

V

L

R

R

P

P

I

L

T

T

Y

L

T

T

I

T

P

P

K
|
K

P

P

M

L

I

V

P

P

I

F

L

C

Q

N

K

K

P

P

V

M

M

I

E

I

F

H

L

Q

L

I

E

E

L

A

L

L

K

K

E

A

H

V

G

G

F

V

K

T

E

E

V

V

M

I

V

L

N

A

V

V

S

A

H

Y

L

R

A

P

Q

E

E

A

I

M

E

K

S

E

Y

Q

F

M

R

D

D

E

G

W

Q

S

R

R

-

K

F

L

G

G

V

V

E

S

V

F

A

V

Y

F

S

S

F

V

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

E

E

E

A
x
P

L
|
L

G

G

S

T

A

A

G

G

G

P

M

L

K

A

R

L

I

A

Q

R

D

D

F

I

S

L

P

M

F

Q

F

D

D

D

D

K

T

P

F

F

V

F

V

V

L

L

C
x
N

G
x
S

D
|
D

A

V

L

T

I

C

D

T

L

F

D

P

L

M

T

Q

E

E

A

L

V

L

R

D

W

F

H

H

K

K

E

A

K

H

G

G

A

G

L

E

A

G

T

T

I

I

V

M

M

V

K

V

T

T

V

-

P

-

R

-

E

-

E

Q

V

W

S

E

S

K

Y

Y

G

G

V

V

-

Y

T

S

D

P

S

Q

D

N

G

Y

R

Q

I

I

Q

E

A

R

F

F

Q

V

E
|
E

K

K

P

P

S

S

V

-

E

-

E

R

A

F

L

L

S

G

T

D

T

R

I

I

N
|
N

T

A

G

G

I

I

Y

Y

I

I

F

F

E

N

P

K

E

S

I

I

L

L

N

D

Y

R

I

I

P

P

S

P

G

-

Q

R

E

R

Y

A

D

S

I

I

G
x
E

G

K

D

E

L

I

F

F

P

P

K

A

L

M

V

A

E

A

T

E

G

G

A

-

P

-

F

-

Y

-

G

-

I

-

S

Q

M

L

D

E

F

G

Q

F

W
|
W

I

M

D
|
D

I

V

G

G

K

Q

V

P

P

K

D

D

Y

Y

-

I

-

L

-

G

-

M

W

T

Q

K

A

F

I

I

R

P

S

S

V

L

L

V

N

H

G

G

E

N

V

R

R

E

N

H

V

T

A

E

I

A

P

V

G

E

K

H

Q

Q

V

-

R

R

P

G

G

G

V

R

Y

F

T

T

G

-

L

-

N

-

V

-

A

-

I

-

D

-

W

-

D

-

S

-

V

-

A

-

I

V

E

I

G

G

P

A

V

S

Y

L

I

I

G

D

G

P

M

S

T

A

R

K

I

I

D

G

E

D

G

G

V

A

R

-

I

V

V

I

G

G

P

P

-

Y

A

A

M

S

I

I

G

G

P

A

N

N

C

C

H

V

I

I

C

G

K

E

G

S

A

C

T

R

I

I

E

D

N

N

S

A

V

A

I

I

F

L

E

E

Y

N

S

S

R

K

L

V

G

G

S

K

G

G

V

T

R

M

L

V

V

S

D

R

K

S

L

I

V

V

-

G

-

W

-

N

-

R

F

I

G

G

R

S

Y

W

C

C

V

H

D

I

R

K

N

D

G

I

N

S

A

V

I

L

D

G

V

D

Q

D

A

V

A

E

A

V

L

K

D

D

W

G

L

V

I

I

T

L

D

I

A

G

R

T

R

K

A

V

E

L

P

P

V

N

Q

K

P

D

S

V

A

G

E

E

H

H

Q

R

binding guanosine-5'-diphosphate-alpha-d-mannose: L6 (= L6), V7 (≠ A7), G9 (= G9), G11 (= G11), K23 (= K23), P83 (≠ A91), L84 (= L92), N108 (≠ C116), S109 (≠ G117), D110 (= D118), E159 (= E170), N170 (= N183), E192 (≠ G206), W209 (= W229), D211 (= D231)
binding magnesium ion: D110 (= D118), D211 (= D231)

7x8kB Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
30% identity, 87% coverage: 1:339/389 of query aligns to 1:328/367 of 7x8kB

query
sites
7x8kB

M

M

K

K

A

A

M

L

I

I

L

L

A

V

A

G

G
|
G

K
x
F

G
|
G

T
|
T

R

R

V

L

R

R

P

P

I

L

T

T

Y

L

T

S

I

F

P

P

K
|
K

P

P

M

L

I

V

P

D

I

F

L

A

Q

N

K

K

P

P

V

M

M

I

E

L

F

H

L

Q

L

I

E

E

L

A

L

L

K

K

E

A

H

V

G

G

F

V

K

D

E

E

V

V

M

V

V

L

N

A

V

I

S

N

H

Y

L

Q

A

P

Q

E

E

V

I

M

E

L

S

N

Y

F

F

L

R

K

D

D

G

F

Q

E

-

T

R

K

F

L

G

E

V

I

E

K

V

I

A

T

Y

C

S

S

F

Q

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

T

E

E

A

P

L

L

G

G

S

T

A

A

G

G

G

P

M

L

K

A

R

L

I

A

Q

R

D

D

F

-

S

-

P

K

F

L

D

D

-

G

-

S

-

G

D

E

T

P

F

F

V

F

V

V

L

L

C

N

G

S

D

D

A

V

L

I

I

S

D

E

L

Y

D

P

L

L

T

K

E

E

A

M

V

L

R

E

W

F

H

H

K

K

E

S

K

H

G

G

A

G

L

E

A

A

T

S

I

I

V

M

M

V

K

T

T

K

V

V

P

-

R

-

E

D

E

E

V

P

S

S

S

K

Y

Y

G

G

V

V

T

M

-

E

D

E

S

S

D

T

G

G

R

R

I

V

Q

E

A

K

F

F

Q

V

E

E

K

K

P

P

S

K

V

L

E

-

A

Y

L

V

S

G

T

N

T

K

I

I

N

N

T

A

G

G

I

I

Y

Y

I

L

F

L

E

N

P

P

E

S

I

V

L

L

N

D

Y

K

I

I

P

-

S

E

G

L

Q

R

E

P

Y

T

D

S

I

I

G

E

G

K

D

E

L

T

F

F

P

P

K

K

L

I

V

A

E

A

T

A

G

Q

A

G

P

-

F

L

Y

Y

G

A

I

M

S

V

M

L

D

P

F

G

Q

F

W

W

I

M

D

D

I

I

G

G

K

Q

V

P

P

R

D

D

Y

Y

W

I

Q

T

A

G

I

L

R

R

S

L

V

Y

L

L

N

D

G

S

E

L

V

R

R

K

N

K

V

S

A

-

I

-

P

P

G

A

K

K

Q

-

V

L

R

T

P

S

G

G

V

P

Y

H

T

I

G

V

L

G

N

N

V

V

A

L

I

V

D

D

W

E

D

T

S

A

V

T

A

I

I

G

E

E

G

G

-

C

-

L

-

I

-

G

-

P

P

D

V

V

Y

A

I

I

G

G

G

P

M

G

T

C

R

I

I

V

D

E

E

S

G

G

V

V

R

R

I

L

V

-

G

S

P

R

A

C

M

T

I

V

G

M

P

R

N

G

C

V

H

R

I

I

C

K

K

K

G

H

A

A

T

C

I

I

E

S

N

S

S

S

V

I

I

I

F

G

E

W

Y

H

S

S

R

T

L

V

G

G

S

Q

G

W

V

A

R

R

L

I

V

E

D

N

K

M

L

T

V

I

F

L

G

G

binding pyrophosphate: G9 (= G9), F10 (≠ K10), G11 (= G11), T12 (= T12), K23 (= K23)

O22287 Mannose-1-phosphate guanylyltransferase 1; GDP-mannose pyrophosphorylase 1; Protein CYTOKINESIS DEFECTIVE 1; Protein EMBRYO DEFECTIVE 101; Protein HYPERSENSITIVE TO AMMONIUM ION 1; Protein SENSITIVE TO OZONE 1; Protein VITAMIN C DEFECTIVE 1; EC 2.7.7.13 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
30% identity, 87% coverage: 1:339/389 of query aligns to 1:328/361 of O22287

query
sites
O22287

M

M

K

K

A

A

M

L

I

I

L
|
L

A
x
V

A

G

G
|
G

K

F

G
|
G

T
|
T

R
|
R

V

L

R

R

P

P

I

L

T

T

Y

L

T

S

I

F

P
|
P

K
|
K

P

P

M

L

I

V

P
x
D

I

F

L

A

Q

N

K

K

P

P

V

M

M

I

E

L

F

H

L

Q

L

I

E

E

L

A

L

L

K

K

E

A

H

V

G

G

F

V

K

D

E

E

V

V

M

V

V

L

N

A

V

I

S

N

H

Y

L

Q

A

P

Q

E

E

V

I

M

E

L

S

N

Y

F

F

L

R

K

D

D

G

F

Q

E

-

T

R

K

F

L

G

E

V

I

E

K

V

I

A

T

Y

C

S

S

F

Q

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

T

E

E

A

P

L

L

G
|
G

S

T

A

A

G

G

G
x
P

M

L

K

A

R

L

I

A

Q

R

D

D

F

-

S

-

P

K

F

L

D

D

-

G

-

S

-

G

D

E

T

P

F

F

V

F

V

V

L

L

C
x
N

G

S

D
|
D

A

V

L

I

I

S

D

E

L

Y

D

P

L

L

T

K

E

E

A

M

V

L

R

E

W

F

H

H

K

K

E

S

K

H

G

G

A

G

L

E

A

A

T

S

I

I

V

M

M

V

K

T

T

K

V

V

P

-

R

-

E

D

E

E

V

P

S

S

S

K

Y

Y

G
|
G

V

V

T

M

-

E

D

E

S

S

D

T

G

G

R

R

I

V

Q

E

A

K

F

F

Q

V

E

E

K

K

P

P

S

K

V

L

E

-

A

Y

L

V

S

G

T

N

T

K

I

I

N
|
N

T

A

G

G

I

I

Y

Y

I

L

F

L

E

N

P

P

E

S

I

V

L

L

N

D

Y

K

I

I

P

-

S

E

G

L

Q

R

E

P

Y

T

D

S

I

I

G

E

G

K

D

E

L

T

F

F

P

P

K

K

L

I

V

A

E

A

T

A

G

Q

A

G

P

-

F

L

Y

Y

G

A

I

M

S

V

M

L

D

P

F

G

Q

F

W

W

I

M

D

D

I

I

G

G

K

Q

V
x
P

P
x
R

D
|
D

Y
|
Y

W
x
I

Q
x
T

A
x
G

I
x
L

R
|
R

S
x
L

V
x
Y

L
|
L

N
x
D

G
x
S

E
x
L

V
x
R

R
x
K

N
x
K

V
x
S

A

-

I

-

P
|
P

G
x
A

K
|
K

Q

-

V
x
L

R
x
T

P
x
S

G
|
G

V
x
P

Y
x
H

T
x
I

G
x
V

L
x
G

N
|
N

V
|
V

A
x
L

I
x
V

D
|
D

W
x
E

D
x
T

S
x
A

V
x
T

A
x
I

I
x
G

E
|
E

G
|
G

-
x
C

-
x
L

-
x
I

-
x
G

-
x
P

P
x
D

V
|
V

Y
x
A

I
|
I

G
|
G

G
x
P

M
x
G

T
x
C

R
x
I

I
x
V

D
x
E

E
x
S

G
|
G

V
|
V

R
|
R

I
x
L

V

-

G
x
S

P
x
R

A
x
C

M
x
T

I
x
V

G
x
M

P
x
R

N
x
G

C
x
V

H
x
R

I
|
I

C
x
K

K
|
K

G
x
H

A
|
A

T
x
C

I
|
I

E
x
S

N
x
S

S
|
S

V
x
I

I
|
I

F
x
G

E
x
W

Y
x
H

S
|
S

R
x
T

L
x
V

G
|
G

S
x
Q

G
x
W

V
x
A

R
|
R

L
x
I

V
x
E

D
x
N

K
x
M

L
x
T

V
x
I

F
x
L

G
|
G

L6 (= L6) binding
V7 (≠ A7) binding
G9 (= G9) binding
G11 (= G11) binding ; mutation to S: In hsn1; reduced enzyme activity, ascorbate concentrations and N-glycosylation, and increased sensitivity to ammonium.
T12 (= T12) binding
R13 (= R13) binding
P22 (= P22) mutation to S: In vtc1-1 and vtc1-2; reduced enzyme activity and ascorbate concentrations, and ozone-sensitive.
K23 (= K23) binding
D27 (≠ P27) mutation to E: Abolishes interaction with CSN5B and subsequent degradation in the dark by the 26S proteasome, and increases ascorbate accumulation in seedlings.
G85 (= G93) binding
P89 (≠ G97) mutation to L: In cyt1-1; deficient in N-glycosylation and cellulose, and embryo lethal.
N109 (≠ C116) binding
D111 (= D118) binding
G146 (= G155) binding
N173 (= N183) binding

Sites not aligning to the query:

223:361 mutation Missing: Reduces catalytic activity 3-fold.

7x8kA Arabidopsis gdp-d-mannose pyrophosphorylase (vtc1) structure (product- bound) (see paper)
30% identity, 87% coverage: 1:339/389 of query aligns to 1:327/365 of 7x8kA

query
sites
7x8kA

M

M

K

K

A

A

M

L

I

I

L

L

A
x
V

A
x
G

G

G

K

F

G

G

T

T

R

R

V

L

R

R

P

P

I

L

T

T

Y

L

T

S

I

F

P

P

K

K

P

P

M

L

I

V

P

D

I

F

L

A

Q

N

K

K

P

P

V

M

M

I

E

L

F

H

L

Q

L

I

E

E

L

A

L

L

K

K

E

A

H

V

G

G

F

V

K

D

E

E

V

V

M

V

V

L

N

A

V

I

S

N

H

Y

L

Q

A

P

Q

E

E

V

I

M

E

L

S

N

Y

F

F

L

R

K

D

D

G

F

Q

E

-

T

R

K

F

L

G

E

V

I

E

K

V

I

A

T

Y

C

S

S

F

Q

E
|
E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

T

E

E

A

P

L

L

G
|
G

S

T

A

A

G

G

G
x
P

M

L

K

A

R

L

I

A

Q

R

D

D

F

-

S

-

P

K

F

L

D

D

-

G

-

S

-

G

D

E

T

P

F

F

V

F

V

V

L

L

C
x
N

G

S

D
|
D

A

V

L

I

I

S

D

E

L

Y

D

P

L

L

T

K

E

E

A

M

V

L

R

E

W

F

H

H

K

K

E

S

K

H

G

G

A

G

L

E

A

A

T

S

I

I

V

M

M

V

K

T

T

K

V

V

P

-

R

-

E

D

E

E

V

P

S

S

S

K

Y

Y

G
|
G

V

V

T

M

-

E

D

E

S

S

D

T

G

G

R

R

I

V

Q

E

A

K

F

F

Q

V

E
|
E

K

K

P

P

S

L

V

Y

E

-

A

-

L

V

S

G

T

N

T

K

I

I

N
|
N

T

A

G
|
G

I

I

Y

Y

I

L

F

L

E

N

P

P

E

S

I

V

L

L

N

D

Y

K

I

I

P

-

S

E

G

L

Q

R

E

P

Y

T

D

S

I

I

G
x
E

G

K

D

E

L

T

F

F

P

P

K

K

L

I

V

A

E

A

T

A

G

Q

A

G

P

-

F

L

Y

Y

G

A

I

M

S

V

M

L

D

P

F

G

Q

F

W

W

I

M

D

D

I

I

G

G

K

Q

V

P

P

R

D

D

Y

Y

W

I

Q

T

A

G

I

L

R

R

S

L

V

Y

L

L

N

D

G

S

E

L

V

R

R

K

N

K

V

S

A

-

I

-

P

P

G

A

K

K

Q

-

V

L

R

T

P

S

G

G

V

P

Y

H

T

I

G

V

L

G

N

N

V

V

A

L

I

V

D

D

W

E

D

T

S

A

V

T

A

I

I

G

E

E

G

G

-

C

-

L

-

I

-

G

-

P

P

D

V

V

Y

A

I

I

G

G

G

P

M

G

T

C

R

I

I

V

D

E

E

S

G

G

V

V

R

R

I

L

V

-

G

S

P

R

A

C

M

T

I

V

G

M

P

R

N

G

C

V

H

R

I

I

C

K

K

K

G

H

A

A

T

C

I

I

E

S

N

S

S

S

V

I

I

I

F

G

E

W

Y

H

S

S

R

T

L

V

G

G

S

Q

G

W

V

A

R

R

L

I

V

E

D

N

K

M

L

T

V

I

F

L

G

G

binding guanosine-5'-diphosphate-alpha-d-mannose: V7 (≠ A7), G8 (≠ A8), E80 (= E79), G85 (= G93), P89 (≠ G97), N109 (≠ C116), D111 (= D118), G146 (= G155), E162 (= E170), N172 (= N183), G174 (= G185), E194 (≠ G206)

4y7vA Structural analysis of muru (see paper)
33% identity, 52% coverage: 1:201/389 of query aligns to 1:188/216 of 4y7vA

query
sites
4y7vA

M

M

K

K

A

A

M

M

I

I

L

L

A

A

G
|
G

K
|
K

G
|
G

T
x
E

R
|
R

V

M

R

R

P

P

I

L

T

T

Y

L

T

H

I

T

P

P

K
|
K

P

P

M

L

I

V

P

P

I

V

L

A

Q

G

K

Q

P

P

V

L

M

I

E

E

F

Y

L

H

L

L

E

R

L

A

L

L

K

A

E

A

H

A

G

G

F

V

K

T

E

E

V

V

M

V

V

I

N

N

V

H

S

A

H

W

L

L

A

G

Q

Q

E

Q

I

I

E

E

S

D

Y

H

F

L

R

G

D

D

G

G

Q

S

R

R

F

F

G

G

V

L

E

S

V

I

A

R

Y

Y

S

S

F

P

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

G

E

E

A

P

L

L

G

E

S

T

A

G

G

G

M

I

K

-

R

-

I

F

Q

K

D

A

F

L

S

P

P

L

F

L

F

G

D

D

D

A

T

P

F

F

V

L

L

V

C
x
N

G

G

D

D

A

V

L

W

I

T

D

D

L

Y

D

D

L

F

T

A

E

-

A

-

V

-

R

R

W

L

H

Q

K

A

E

P

K

L

G

Q

A

G

L

L

A

A

T

H

I

L

V

V

M

L

K

V

T

D

V

N

P

P

-

G

R

R

E

G

E
x
D

V

F

S

R

S

L

Y

V

G

G

V

E

V

Q

V

V

T

V

D

D

S

G

D

D

G

D

R

G

I

T

Q

L

A

T

F
|
F

Q

S

E

G

K

I

P

S

S

V

V

L

E

H

E

P

A

A

L

L

S

F

T

E

T

G

I

C

N

Q

T

A

G

G

I

A

Y

F

I

K

F

L

E

A

P

P

E

L

I

L

L

R

N

Q

Y

A

I

M

P

A

S

A

G

G

Q

K

binding imidodiphosphoric acid: G9 (= G9), K10 (= K10), G11 (= G11), E12 (≠ T12), R13 (= R13), K23 (= K23)
binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: N105 (≠ C116), D137 (≠ E150), F155 (= F168)

Sites not aligning to the query:

binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: 198, 200

4y7uA Structural analysis of muru (see paper)
31% identity, 60% coverage: 1:235/389 of query aligns to 1:209/224 of 4y7uA

query
sites
4y7uA

M

M

K

K

A

A

M

M

I

I

L
|
L

A
|
A

G
|
G

K

K

G
|
G

T
x
E

R
|
R

V

M

R

R

P

P

I

L

T

T

Y

L

T

H

I

T

P

P

K
|
K

P

P

M

L

I

V

P

P

I

V

L

A

Q

G

K

Q

P

P

V

L

M

I

E

E

F

Y

L

H

L

L

E

R

L

A

L

L

K

A

E

A

H

A

G

G

F

V

K

T

E

E

V

V

M

V

V

I

N

N

V

H

S

A

H

W

L

L

A

G

Q

Q

E

Q

I

I

E

E

S

D

Y

H

F

L

R

G

D

D

G

G

Q

S

R

R

F

F

G

G

V

L

E

S

V

I

A

R

Y

Y

S

S

F

P

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

G

E

E

A

P

L

L

G
x
E

S

T

A

G

G

G

M

I

K

-

R

-

I

F

Q

K

D

A

F

L

S

P

P

L

F

L

F

G

D

D

D

A

T

P

F

F

V

L

L

V

C
x
N

G

G

D
|
D

A

V

L

W

I

T

D

D

L

Y

D

D

L

F

T

A

E

-

A

-

V

-

R

R

W

L

H

Q

K

A

E

P

K

L

G

Q

A

G

L

L

A

A

T

H

I

L

V

V

M

L

K

V

T

D

V

N

P

P

-

G

-

H

-

G

R

R

E

G

E
x
D

V

F

S

R

S

L

Y

V

G

G

V

E

V

Q

V

V

T

V

D

D

S

G

D

D

G

-

R

-

I

-

Q

-

A

-

F

-

Q

-

E

-

K

-

P

-

S

-

V

-

E

-

E

D

A

A

L

P

S

G

T

T

T

L

I

T

N
x
F

T

S

G

G

I

I

Y

S

I

V

F

L

E

H

P

P

E

A

I

L

L

F

N

E

Y

G

I

C

P

Q

S

A

G

G

Q

A

E

F

Y

-

D

-

I

-

G

-

D

K

L

L

F

A

P

P

K

L

L

L

V

R

E

Q

T

A

G

M

A

A

P

A

-

G

-

K

F

V

Y

S

G

G

I

E

S

H

M

Y

D

R

F

G

Q

H

W
|
W

I

V

D
|
D

I

V

G

G

K

T

V

L

binding 5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]uridine: L6 (= L6), A7 (= A7), A8 (= A8), G9 (= G9), G11 (= G11), E12 (≠ T12), R13 (= R13), K23 (= K23), E84 (≠ G93), D107 (= D118)
binding 2-acetamido-3-O-[(1R)-1-carboxyethyl]-2-deoxy-1-O-phosphono-alpha-D-glucopyranose: N105 (≠ C116), D140 (≠ E150), F160 (≠ N183), W203 (= W229), D205 (= D231)
binding magnesium ion: D107 (= D118), D205 (= D231)

Q88QT2 N-acetylmuramate alpha-1-phosphate uridylyltransferase; MurNAc-1P uridylyltransferase; MurNAc-alpha-1P uridylyltransferase; EC 2.7.7.99 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
31% identity, 60% coverage: 1:235/389 of query aligns to 1:209/223 of Q88QT2

query
sites
Q88QT2

M

M

K

K

A

A

M

M

I

I

L

L

A

A

G

G

K

K

G
|
G

T
x
E

R
|
R

V

M

R

R

P

P

I

L

T

T

Y

L

T

H

I

T

P

P

K
|
K

P

P

M

L

I

V

P

P

I

V

L

A

Q

G

K

Q

P

P

V

L

M

I

E

E

F

Y

L

H

L

L

E

R

L

A

L

L

K

A

E

A

H

A

G

G

F

V

K

T

E

E

V

V

M

V

V

I

N

N

V

H

S

A

H

W

L

L

A

G

Q

Q

E

Q

I

I

E

E

S

D

Y

H

F

L

R

G

D

D

G

G

Q

S

R

R

F

F

G

G

V

L

E

S

V

I

A

R

Y

Y

S

S

F

P

E

E

G

-

R

-

I

-

E

-

D

-

G

-

Q

-

L

-

I

-

G

G

E

E

A

P

L

L

G

E

S

T

A

G

G

G

M

I

K

-

R

-

I

F

Q

K

D

A

F

L

S

P

P

L

F

L

F

G

D

D

D

A

T

P

F

F

V

L

L

V

C
x
N

G

G

D
|
D

A

V

L

W

I

T

D

D

L

Y

D

D

L

F

T

A

E

-

A

-

V

-

R

R

W

L

H

Q

K

A

E

P

K

L

G

Q

A

G

L

L

A

A

T

H

I

L

V

V

M

L

K

V

T

D

V

N

P

P

-

G

-

H

-

G

R

R

E

G

E
x
D

V

F

S

R

S

L

Y

V

G

G

V

E

V

Q

V

V

T

V

D

D

S

G

D

D

G

-

R

-

I

-

Q

-

A

-

F

-

Q

-

E

-

K

-

P

-

S

-

V

-

E

-

E

D

A

A

L

P

S

G

T

T

T

L

I

T

N

F

T

S

G

G

I

I

Y

S

I

V

F

L

E

H

P

P

E

A

I

L

L

F

N

E

Y

G

I

C

P

Q

S

A

G

G

Q

A

E

F

Y

-

D

-

I

-

G

-

D

K

L

L

F

A

P

P

K

L

L

L

V

R

E

Q

T

A

G

M

A

A

P

A

-

G

-

K

F

V

Y

S

G

G

I

E

S

H

M

Y

D

R

F

G

Q

H

W

W

I

V

D
|
D

I

V

G

G

K

T

V

L

GER 11:13 (≠ GTR 11:13) binding
K23 (= K23) binding
N105 (≠ C116) binding
D107 (= D118) binding
D140 (≠ E150) binding
D205 (= D231) binding ; binding

5z09A St0452(y97n)-utp binding form (see paper)
26% identity, 87% coverage: 1:338/389 of query aligns to 1:301/401 of 5z09A

query
sites
5z09A

M

M

K

K

A

A

M

F

I

I

L
|
L

A
|
A

G
|
G

K
x
S

G
|
G

T
x
E

R
|
R

V

L

R

E

P

P

I

I

T

T

Y

H

T

T

I

R

P

P

K

K

P

A

M

F

I

V

P

P

I

I

L

L

Q

S

K

K

P

P

V

L

M

I

E

E

F

Y

L

Q

L

I

E

E

L

Y

L

L

K

R

E

K

H

C

G

G

F

I

K

R

E

D

V

I

M

T

V

V

N

I

V

V

S

S

H

S

L

K

A

N

Q

K

E

E

I

-

E

-

S

-

Y

Y

F

F

R

E

D

K

G

K

Q

L

R

K

F

-

G

-

V

-

E

-

V

-

A

E

Y

I

S

S

F

I

E

V

G

T

R
x
Q

I

K

E

D

D

D

G

I

Q

K

L
x
G

I

T

G

G

E

A

A

A

L

I

G

L

S

S

A

A

G

K

G

-

M

-

K

-

R

-

I

-

Q

-

D

-

F

-

S

-

P

-

F

-

F

F

D

N

D

D

T

E

F

A

V

L

V

I

L

I

C

N

G
|
G

D
|
D

A

L

L

F

I

-

D

-

L

-

D

-

L

-

T

-

E

-

A

-

V

-

R

-

W

F

H

S

K

N

E

E

K

K

G

E

A

I

L

C

A

N

T

I

I

I

V

T

M

L

K

K

T

E

-

N

-

A

-

I

-

G

V

V

P

K

R

V

E

S

E

N

V

P

S

K

S

D

Y

Y

G

G

V

V

V

L

V

V

T

L

D

D

S

N

D

Q

G

N

R

N

I

L

Q

S

A

K

F

I

Q

I

E

E

K

K

P

P

S

E

V

I

E

P

A

-

L

-

S

S

T

N

T

L

I

I

N

N

T

A

G

G

I

I

Y

Y

I

K

F

L

E

N

P

S

E

D

I

I

L

F

N

T

Y

Y

I

L

P

D

S

K

G

I

Q

S

E

I

Y

S

D

E

I

R

G

G

G

E

D

L

L

E

F

L

P

T

K

D

L

A

V

I

E

N

T

L

G

M

A

A

P

K

F

D

Y

H

G

R

I

V

S

K

M

V

-

I

-

E

-

Y

D

E

F

G

Q

Y

W

W

I

M

D

D

I

I

G

G

K

K

V

P

P

-

D

-

Y

-

W

W

Q

-

A

-

I

-

R

-

S

-

V

-

L

-

N

-

G

-

E

-

V

-

R

-

N

N

V

I

A

I

I

D

P

V

G

N

K

K

Q

W

V

A

R

L

P

D

G

N

V

L

Y

V

T

F

G

S

L

Q

N

N

V

L

A

G

I

N

D

V

W

E

D

D

S

N

V

V

A

K

I

I

E

K

G

G

P

K

V

V

Y

I

I

I

G

E

M

D

T

A

R

E

I

I

D

K

E

S

G

G

V

T

R

Y

I

I

V

E

G

G

P

P

A

V

M

Y

I

I

G

G

P

K

N

G

C

S

H

E

I

I

C

G

K

P

G

N

A

S

T

Y

I

L

E

R

-

P

N

Y

S

T

V

I

I

L

F

V

E

E

Y

K

S

N

R

K

L

I

G

G

S

A

G

S

V

V

R

E

L

V

V

K

D

E

K

S

L

V

V

I

F

M

binding uridine 5'-triphosphate: L6 (= L6), A7 (= A7), A8 (= A8), G9 (= G9), S10 (≠ K10), G11 (= G11), E12 (≠ T12), R13 (= R13), Q73 (≠ R81), G79 (≠ L87), G98 (= G117), D99 (= D118)

2ggqA Complex of hypothetical glucose-1-phosphate thymidylyltransferase from sulfolobus tokodaii
26% identity, 87% coverage: 1:338/389 of query aligns to 1:301/401 of 2ggqA

query
sites
2ggqA

M

M

K

K

A

A

M

F

I

I

L
|
L

A

A

A
|
A

G
|
G

K
x
S

G
|
G

T
x
E

R
|
R

V

L

R

E

P

P

I

I

T

T

Y

H

T

T

I

R

P

P

K
|
K

P

A

M

F

I

V

P

P

I

I

L

L

Q

S

K

K

P

P

V

L

M

I

E

E

F

Y

L

Q

L

I

E

E

L

Y

L

L

K

R

E

K

H

C

G

G

F

I

K

R

E

D

V

I

M

T

V

V

N

I

V

V

S

S

H

S

L

K

A

N

Q

K

E

E

I

-

E

-

S

-

Y

Y

F

F

R

E

D

K

G

K

Q

L

R

K

F

-

G

-

V

-

E

-

V

-

A

E

Y

I

S

S

F

I

E

V

G

T

R
x
Q

I

K

E

D

D

D

G

I

Q

K

L
x
G

I

T

G

G

E

A

A
|
A

L

I

G

L

S

S

A

A

G

K

G

-

M

-

K

-

R

-

I

-

Q

-

D

-

F

-

S

-

P

-

F

-

F

F

D

N

D

D

T

E

F

A

V

L

V

I

L

I

C

Y

G

G

D

D

A

L

L

F

I

-

D

-

L

-

D

-

L

-

T

-

E

-

A

-

V

-

R

-

W

F

H

S

K

N

E

E

K

K

G

E

A

I

L

C

A

N

T

I

I

I

V

T

M

L

K

K

T

E

-

N

-

A

-

I

-

G

V

V

P

K

R

V

E

S

E

N

V

P

S

K

S

D

Y

Y

G

G

V

V

V

L

V

V

T

L

D

D

S

N

D

Q

G

N

R

N

I

L

Q

S

A

K

F

I

Q

I

E

E

K

K

P

P

S

E

V

I

E

P

A

-

L

-

S

S

T

N

T

L

I

I

N

N

T

A

G

G

I

I

Y

Y

I

K

F

L

E

N

P

S

E

D

I

I

L

F

N

T

Y

Y

I

L

P

D

S

K

G

I

Q

S

E

I

Y

S

D

E

I
x
R

G

G

G
x
E

D

L

L

E

F

L

P

T

K

D

L

A

V

I

E

N

T

L

G

M

A

A

P

K

F

D

Y

H

G

R

I

V

S

K

M

V

-

I

-

E

-

Y

D

E

F

G

Q

Y

W

W

I

M

D

D

I

I

G

G

K

K

V

P

P

-

D

-

Y

-

W

W

Q

-

A

-

I

-

R

-

S

-

V

-

L

-

N

-

G

-

E

-

V

-

R

-

N

N

V

I

A

I

I

D

P

V

G

N

K

K

Q

W

V

A

R

L

P

D

G

N

V

L

Y

V

T

F

G

S

L

Q

N

N

V

L

A

G

I

N

D

V

W

E

D

D

S

N

V

V

A

K

I

I

E

K

G

G

P

K

V

V

Y

I

I

I

G

E

M

D

T

A

R

E

I

I

D

K

E

S

G

G

V

T

R

Y

I

I

V

E

G

G

P

P

A

V

M

Y

I

I

G

G

P

K

N

G

C

S

H

E

I

I

C

G

K

P

G

N

A

S

T

Y

I

L

E

R

-

P

N

Y

S

T

V

I

I

L

F

V

E

E

Y

K

S

N

R

K

L

I

G

G

S

A

G

S

V

V

R

E

L

V

V

K

D

E

K

S

L

V

V

I

F

M

active site: R13 (= R13)
binding thymidine-5'-triphosphate: L6 (= L6), A8 (= A8), G9 (= G9), S10 (≠ K10), G11 (= G11), E12 (≠ T12), R13 (= R13), K23 (= K23), Q73 (≠ R81), G79 (≠ L87), A83 (= A91), R179 (≠ I205), E181 (≠ G207)

Sites not aligning to the query:

binding thymidine-5'-triphosphate: 344

Q975F9 Bifunctional sugar-1-phosphate nucleotidylyltransferase/acetyltransferase; EC 2.7.7.24; EC 2.7.7.9; EC 2.7.7.83; EC 2.7.7.23; EC 2.3.1.276; EC 2.3.1.157 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
26% identity, 87% coverage: 1:338/389 of query aligns to 1:301/401 of Q975F9

query
sites
Q975F9

M

M

K

K

A

A

M

F

I

I

L

L

A

A

A
|
A

G
|
G

K
x
S

G
|
G

T
x
E

R
|
R

V

L

R

E

P

P

I

I

T

T

Y

H

T

T

I

R

P

P

K

K

P

A

M

F

I

V

P

P

I

I

L

L

Q

S

K

K

P

P

V

L

M

I

E

E

F

Y

L

Q

L

I

E

E

L

Y

L

L

K

R

E

K

H

C

G

G

F

I

K

R

E

D

V

I

M

T

V

V

N

I

V

V

S

S

H

S

L

K

A

N

Q

K

E

E

I

-

E

-

S

-

Y

Y

F

F

R

E

D

K

G

K

Q

L

R

K

F

-

G

-

V

-

E

-

V

-

A

E

Y

I

S

S

F

I

E

V

G

T

R
x
Q

I

K

E

D

D

D

G

I

Q

K

L
x
G

I
x
T

G

G

E

A

A

A

L

I

G

L

S

S

A

A

G

K

G

-

M

-

K

-

R

-

I

-

Q

-

D

-

F

-

S

-

P

-

F

-

F

F

D

N

D

D

T

E

F

A

V

L

V

I

L

I

C
x
Y

G

G

D

D

A

L

L

F

I

-

D

-

L

-

D

-

L

-

T

-

E

-

A

-

V

-

R

-

W

F

H

S

K

N

E

E

K

K

G

E

A

I

L

C

A

N

T

I

I

I

V

T

M

L

K

K

T

E

-

N

-

A

-

I

-

G

V

V

P

K

R

V

E

S

E

N

V

P

S

K

S

D

Y

Y

G

G

V

V

V

L

V

V

T

L

D

D

S

N

D

Q

G

N

R

N

I

L

Q

S

A

K

F

I

Q

I

E
|
E

K

K

P

P

S

E

V

I

E

P

A

-

L

-

S

S

T

N

T

L

I

I

N

N

T

A

G

G

I

I

Y

Y

I

K

F

L

E

N

P

S

E

D

I

I

L

F

N

T

Y

Y

I

L

P

D

S

K

G

I

Q

S

E

I

Y

S

D

E

I

R

G

G

G

E

D

L

L

E

F

L

P

T

K

D

L

A

V

I

E

N

T

L

G

M

A

A

P

K

F

D

Y

H

G

R

I

V

S

K

M

V

-

I

-

E

-

Y

D

E

F

G

Q

Y

W

W

I

M

D

D

I

I

G

G

K

K

V

P

P

-

D

-

Y

-

W

W

Q

-

A

-

I

-

R

-

S

-

V

-

L

-

N

-

G

-

E

-

V

-

R

-

N

N

V

I

A

I

I

D

P

V

G

N

K

K

Q

W

V

A

R

L

P

D

G

N

V

L

Y

V

T

F

G

S

L

Q

N

N

V

L

A

G

I

N

D

V

W

E

D

D

S

N

V

V

A

K

I

I

E

K

G

G

P

K

V

V

Y

I

I

I

G

E

M

D

T

A

R

E

I

I

D

K

E

S

G

G

V

T

R

Y

I

I

V

E

G

G

P

P

A

V

M

Y

I

I

G

G

P

K

N

G

C

S

H

E

I

I

C

G

K

P

G

N

A

S

T

Y

I

L

E

R

-

P

N

Y

S

T

V

I

I

L

F

V

E

E

Y

K

S

N

R

K

L

I

G

G

S

A

G

S

V

V

R

E

L

V

V

K

D

E

K

S

L

V

V

I

F

M

AGSGER 8:13 (≠ AGKGTR 8:13) binding
Q73 (≠ R81) binding
G79 (≠ L87) binding
T80 (≠ I88) mutation T->A,G,Q: Increases both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->D,H: Decrease in GlcNAc-1-P UTase activity but increase in Glc-1-P UTase activity.; mutation T->E,K,L,M,R,W,Y: Strong decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.; mutation T->F,I: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation T->N,S: Strong increase in GlcNAc-1-P UTase activity and decrease in Glc-1-P UTase activity.; mutation to N: Loss of GlcNAc-1-P UTase activity; when associated with V-97.
Y97 (≠ C116) mutation Y->A,D,F,G,I,K,T,V: Increases GlcNAc-1-P UTase activity. Decreases Glc-1-P UTase activity.; mutation Y->C,E,P,R,W: Decreases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation Y->H,L,M,N,Q,S: Increases GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation to V: Loss of GlcNAc-1-P UTase activity; when associated with N-80.
E146 (= E170) mutation E->A,C,F,G,I,K,L,M,P,Q,R,V,W,Y: Loss of both GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->D,N: Decrease in GlcNAc-1-P UTase and Glc-1-P UTase activities.; mutation E->H,S,T: Decrease in GlcNAc-1-P UTase activity and loss of Glc-1-P UTase activity.

Sites not aligning to the query:

308 H→A: Strong decrease in GalN-1-P AcTase activity and almost loss of GlcN-1-P AcTase activity.
311 Y→A: Strong decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
331 N→A: Strong decrease in GalN-1-P AcTase activity and decrease in GlcN-1-P AcTase activity.
337 K→A: Slight decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
340 K→A: Decrease in GalN-1-P AcTase activity and increase in GlcN-1-P AcTase activity.
391:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 38% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 16.8 times. Significantly affects the thermostability of the entire protein.
397:401 mutation Missing: No change in GlcNAc-1-P UTase activity. Shows 20% less GalN-1-P AcTase activity than the wild-type, but increases GlcN-1-P AcTase activity 4.8 times. Does not affect thermostability.

P0A6V1 Glucose-1-phosphate adenylyltransferase; ADP-glucose pyrophosphorylase; ADPGlc PPase; ADP-glucose synthase; EC 2.7.7.27 from Escherichia coli (strain K12) (see 11 papers)
27% identity, 61% coverage: 3:241/389 of query aligns to 22:286/431 of P0A6V1

query
sites
P0A6V1

A

A

M

L

I

I

L

L

A

A

A

G

G

G

K

R

G

G

T

T

R

R

V

L

R

K

P

D

I

L

T

T

Y

N

T
x
K

I
x
R

P

A

K

K

P

P

M
x
A

I

V

P
x
H

I

F

L

G

Q

G

K

K

-

F

P
x
R

V

I

M

I

E

D

F

F

L

A

L

L

E

S

L

N

L

C

K

I

E

N

H

S

G

G

F

I

K
x
R

E

R

V

M

M

G

V

V

N

I

V

T

S
x
Q

H

Y

L

Q

A

S

Q

H

E

T

I

L

E

V

S

Q

Y

H

F

I

R

Q

D

R

G

G

Q

W

R

S

F

F

G

F

V

N

E

E

V

E

A

M

Y

N

S

E

F

F

E

V

G

D

R

L

I

L

E

P

D

A

G

Q

Q

Q

-

R

L

M

I

K

G

G

E

E

A

N

L
x
W

-
x
Y

-

R

G

G

S

T

A

A

G

D

G

A

M

V

K

T

R

Q

I

N

Q

L

D

D

F

I

S

I

P

R

F
x
R

F

Y

D

K

D

A

T

E

F

Y

V

V

-

I

L

L

C

A

G

G

D

D

A

H

L

I

I

Y

D

K

L

Q

D

D

L

Y

T

S

E

R

A

M

V

L

R

I

W

D

H

H

K

V

E

E

K

K

G

G

A

A

L

R

A

C

T

T

I

V

V

A

M

C

K

M

T

P

V

V

P

P

R

I

E

E

V

A

S

S

S

A

Y

F

G

G

V

V

V

M

V

A

T

V

D

D

S

E

D

N

G

D

R

K

I

I

Q

I

A

E

F

F

Q

V

E

E

K
|
K

P

P

S

A

V

N

E

P

A

S

L

M

-

P

-

N

-

D

-

P

-

S

S

K

T

S

T

L

I

A

N

S

T

M

G

G

I

I

Y

Y

I

V

F

F

E

D

P

A

E

D

I

Y

L

L

N

Y

Y

E

I

L

-

L

-

E

-

D

-

R

P

D

S

E

G

N

Q

S

E

S

Y

H

D

D

I

F

G

G

G

K

D

D

L

L

F

I

P

P

K

K

L

I

V

T

E

E

T

A

G

G

A

L

-

A

-

Y

-

A

-

H

-

P

-

F

P

P

F

L

Y

S

G

C

I

V

S

Q

M

S

D

D

F

P

Q

D

-

A

-

E

-

P

-

Y

W

W

I

R

D

D

I

V

G

G

K

T

V

L

P

E

D

A

Y

Y

W

W

Q

K

A

A

K39 (≠ T20) binding ; mutation to E: The level of activation by pyridoxal phosphate and fructose-1,6-phosphate is only approximately 2-fold compared to activation of 15- to 28-fold respectively, for the wild-type. NADPH is unable to activate the mutant enzyme.
R40 (≠ I21) binding
A44 (≠ M25) to T: in SG14 mutant; lower apparent affinities for substrates, fructose-1,6-bisphosphate and AMP
H46 (≠ P27) binding
R52 (≠ P32) binding
R67 (≠ K47) to C: in CL1136 mutant; less dependent on the allosteric activator, fructose-1,6-bisphosphate, for activity and less sensitive to inhibition by AMP
Q74 (≠ S54) mutation to A: Insensitive to activation by fructose-1,6-bisphosphate, but still binds fructose-1,6-bisphosphate with similar affinity as the wild-type. AMP causes similar inhibition on the wild-type and mutant.; mutation to E: Insensitive to activation by fructose-1,6-bisphosphate.; mutation to N: The enzyme is activated 35-fold by fructose-1,6-bisphosphate.
W113 (≠ L92) mutation to A: Insensitive to activation by fructose-1,6-bisphosphate, but still binds fructose-1,6-bisphosphate, with similar affinity as the wild-type. AMP causes similar inhibition on the wild-type and mutant.; mutation to L: The enzyme is activated only 3-fold by fructose-1,6-bisphosphate.; mutation to Y: The enzyme is activated 15-fold by fructose-1,6-bisphosphate.
Y114 (vs. gap) mutation to F: Shows a decrease of affinity for the substrates and less than 2-fold activation by fructose 1,6-bisphosphate in the ADP-glucose synthesis direction. In contrast, in the pyrophosphorolysis direction, the mutant shoqws about a 30-fold activation by fructose 1,6-bisphosphate.
R130 (≠ F107) binding
K195 (= K171) mutation K->E,I,H,R: Decrease of the affinity for alpha-D-glucose 1-phosphate, but no loss in adenylyltransferase activity.; mutation to Q: 600-fold decrease of the affinity for alpha-D-glucose 1-phosphate compared to the wild-type. In contrast, kinetic constants for ATP, magnesium and fructose-1,6-bisphosphate are similar in mutant and wild-type cases. The catalytic efficiency is 2-fold lower in the mutant.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
295 P → S: in SG5 mutant
336 G → D: in 618 mutant; causes lowered affinity for AMP
370 binding
386 binding
419:423 binding
429:431 binding

6r8uA Escherichia coli agpase in complex with amp.
27% identity, 61% coverage: 3:241/389 of query aligns to 16:280/425 of 6r8uA

query
sites
6r8uA

A

A

M

L

I

I

L

L

A

A

A

G

G

G

K

R

G

G

T

T

R

R

V

L

R

K

P

D

I

L

T

T

Y

N

T

K

I
x
R

P

A

K

K

P

P

M

A

I

V

P
x
H

I

F

L

G

Q

G

K

K

-

F

P
x
R

V

I

M

I

E

D

F

F

L

A

L

L

E

S

L

N

L

C

K

I

E

N

H

S

G

G

F

I

K

R

E

R

V

M

M

G

V

V

N

I

V

T

S

Q

H

Y

L

Q

A

S

Q

H

E
x
T

I

L

E

V

S

Q

Y

H

F

I

R

Q

D

R

G

G

Q

W

R

S

F

F

G

F

V

N

E

E

V

E

A

M

Y

N

S

E

F

F

E

V

G

D

R

L

I

L

E

P

D

A

G

Q

Q

Q

-

R

L

M

I

K

G

G

E

E

A

N

L

W

-

Y

-

R

G

G

S

T

A

A

G

D

G

A

M

V

K

T

R

Q

I

N

Q

L

D

D

F

I

S

I

P

R

F

R

F

Y

D

K

D

A

T

E

F

Y

V

V

-

I

L

L

C

A

G

G

D

D

A

H

L

I

I

Y

D

K

L

Q

D

D

L

Y

T

S

E

R

A

M

V

L

R

I

W

D

H

H

K

V

E

E

K

K

G

G

A

A

L

R

A

C

T

T

I

V

V

A

M

C

K

M

T

P

V

V

P

P

R

I

E

E

V

A

S

S

S

A

Y

F

G

G

V

V

V

M

V

A

T

V

D

D

S

E

D

N

G

D

R

K

I

I

Q

I

A

E

F

F

Q

V

E

E

K

K

P

P

S

A

V

N

E

P

A

S

L

M

-

P

-

N

-

D

-

P

-

S

S

K

T

S

T

L

I

A

N

S

T

M

G

G

I

I

Y

Y

I

V

F

F

E

D

P

A

E

D

I

Y

L

L

N

Y

Y

E

I

L

-

L

-

E

-

D

-

R

P

D

S

E

G

N

Q

S

E

S

Y

H

D

D

I

F

G

G

G

K

D

D

L

L

F

I

P

P

K

K

L

I

V

T

E

E

T

A

G

G

A

L

-

A

-

Y

-

A

-

H

-

P

-

F

P

P

F

L

Y

S

G

C

I

V

S

Q

M

S

D

D

F

P

Q

D

-

A

-

E

-

P

-

Y

W

W

I

R

D

D

I

V

G

G

K

T

V

L

P

E

D

A

Y

Y

W

W

Q

K

A

A

binding adenosine monophosphate: R34 (≠ I21), H40 (≠ P27), R46 (≠ P32), T73 (≠ E59)

Sites not aligning to the query:

binding adenosine monophosphate: 364, 380, 381, 413

5l6vA Crystal structure of e. Coli adp-glucose pyrophosphorylase (agpase) in complex with a negative allosteric regulator adenosine monophosphate (amp) - agpase Amp (see paper)
27% identity, 61% coverage: 3:241/389 of query aligns to 14:278/422 of 5l6vA

query
sites
5l6vA

A

A

M

L

I

I

L

L

A

A

A

G

G

G

K

R

G

G

T

T

R

R

V

L

R

K

P

D

I

L

T

T

Y

N

T
x
K

I
x
R

P

A

K

K

P

P

M

A

I

V

P
x
H

I

F

L

G

Q

G

K

K

-

F

P
x
R

V

I

M

I

E

D

F

F

L

A

L

L

E

S

L

N

L

C

K

I

E

N

H

S

G

G

F

I

K

R

E

R

V

M

M

G

V

V

N

I

V

T

S

Q

H

Y

L

Q

A

S

Q

H

E

T

I

L

E

V

S

Q

Y

H

F

I

R

Q

D

R

G

G

Q

W

R

S

F

F

G

F

V

N

E

E

V

E

A

M

Y

N

S

E

F

F

E

V

G

D

R

L

I

L

E

P

D

A

G

Q

Q

Q

-

R

L

M

I

K

G

G

E

E

A

N

L

W

-

Y

-

R

G

G

S

T

A

A

G

D

G

A

M

V

K

T

R

Q

I

N

Q

L

D

D

F

I

S

I

P

R

F

R

F

Y

D

K

D

A

T

E

F

Y

V

V

-

I

L

L

C

A

G

G

D

D

A
x
H

L

I

I

Y

D

K

L

Q

D

D

L

Y

T

S

E

R

A

M

V

L

R

I

W

D

H

H

K

V

E

E

K

K

G

G

A

A

L

R

A

C

T

T

I

V

V

A

M

C

K

M

T

P

V

V

P

P

R

I

E

E

V

A

S

S

S

A

Y
x
F

G
|
G

V

V

V

M

V

A

T

V

D

D

S

E

D

N

G

D

R

K

I

I

Q

I

A

E

F

F

Q

V

E
|
E

K
|
K

P

P

S

A

V

N

E

P

A

S

L

M

-

P

-

N

-

D

-

P

-

S

S

K

T

S

T

L

I

A

N
x
S

T

M

G

G

I

I

Y

Y

I

V

F

F

E

D

P

A

E

D

I

Y

L

L

N

Y

Y

E

I

L

-

L

-

E

-

D

-

R

P

D

S

E

G

N

Q

S

E

S

Y

H

D

D

I

F

G

G

G

K

D

D

L

L

F

I

P

P

K

K

L

I

V

T

E

E

T

A

G

G

A

L

-

A

-

Y

-

A

-

H

-

P

-

F

P

P

F

L

Y

S

G

C

I

V

S

Q

M

S

D

D

F

P

Q

D

-

A

-

E

-

P

-

Y

W

W

I

R

D

D

I

V

G

G

K

T

V

L

P

E

D

A

Y

Y

W

W

Q

K

A

A

binding adenosine monophosphate: K31 (≠ T20), R32 (≠ I21), H38 (≠ P27), R44 (≠ P32)
binding beta-D-fructofuranose: H135 (≠ A119), K187 (= K171)
binding alpha-D-glucopyranose: H135 (≠ A119), F170 (≠ Y154), G171 (= G155), E186 (= E170), S204 (≠ N183)

Sites not aligning to the query:

binding adenosine monophosphate: 362, 378, 379

5l6sC Crystal structure of e. Coli adp-glucose pyrophosphorylase (agpase) in complex with a positive allosteric regulator beta-fructose-1,6- diphosphate (fbp) - agpase Fbp (see paper)
27% identity, 61% coverage: 3:241/389 of query aligns to 9:273/418 of 5l6sC

query
sites
5l6sC

A

A

M

L

I

I

L

L

A

A

A

G

G

G

K

R

G

G

T

T

R

R

V

L

R

K

P

D

I

L

T

T

Y

N

T

K

I

R

P

A

K

K

P

P

M

A

I

V

P

H

I

F

L

G

Q

G

K

K

-

F

P

R

V

I

M

I

E

D

F

F

L

A

L

L

E

S

L

N

L

C

K

I

E

N

H

S

G

G

F

I

K

R

E

R

V

M

M

G

V

V

N

I

V

T

S

Q

H

Y

L

Q

A

S

Q

H

E

T

I

L

E

V

S

Q

Y

H

F

I

R

Q

D

R

G

G

Q

W

R

S

F

F

G

F

V

N

E

E

V

E

A

M

Y

N

S

E

F

F

E

V

G

D

R

L

I

L

E

P

D

A

G

Q

Q

Q

-

R

L

M

I

K

G

G

E

E

A

N

L

W

-

Y

-

R

G

G

S

T

A

A

G

D

G

A

M

V

K

T

R

Q

I

N

Q

L

D

D

F

I

S

I

P

R

F

R

F

Y

D

K

D

A

T

E

F

Y

V

V

-

I

L

L

C

A

G

G

D

D

A

H

L

I

I

Y

D

K

L

Q

D

D

L

Y

T

S

E

R

A

M

V

L

R

I

W

D

H

H

K

V

E

E

K

K

G

G

A

A

L

R

A

C

T

T

I

V

V

A

M

C

K

M

T

P

V

V

P

P

R

I

E

E

V

A

S

S

S

A

Y

F

G

G

V

V

V

M

V

A

T

V

D

D

S

E

D

N

G

D

R

K

I

I

Q

I

A

E

F

F

Q

V

E

E

K

K

P

P

S

A

V

N

E

P

A

S

L

M

-

P

-

N

-

D

-

P

-

S

S

K

T

S

T

L

I

A

N

S

T

M

G

G

I

I

Y

Y

I

V

F

F

E

D

P

A

E

D

I

Y

L

L

N

Y

Y

E

I

L

-

L

-

E

-

D

-

R

P

D

S

E

G

N

Q

S

E

S

Y

H

D

D

I

F

G

G

G

K

D

D

L

L

F

I

P

P

K

K

L

I

V

T

E

E

T

A

G

G

A

L

-

A

-

Y

-

A

-

H

-

P

-

F

P

P

F

L

Y

S

G

C

I

V

S

Q

M

S

D

D

F

P

Q

D

-

A

-

E

-

P

-

Y

W

W

I

R

D

D

I

V

G

G

K

T

V

L

P

E

D

A

Y

Y

W

W

Q

K

A

A

Sites not aligning to the query:

binding 1,6-di-O-phosphono-beta-D-fructofuranose: 406, 407, 410, 415, 416, 417

Q8Z5I4 Glucose-1-phosphate cytidylyltransferase; CDP-glucose pyrophosphorylase; EC 2.7.7.33 from Salmonella typhi (see 2 papers)
28% identity, 51% coverage: 1:197/389 of query aligns to 1:200/257 of Q8Z5I4

query
sites
Q8Z5I4

M

M

K

K

A

A

M

V

I

I

L

L

A

A

A

G

G

G

K

L

G

G

T

T

R

R

V

L

R

S

P

E

I

E

T

T

Y

I

T

V

I

K

P

P

K
|
K

P

P

M

M

I

V

P

E

I

I

L

G

Q

G

K

K

P

P

V

I

M

L

E

W

F

H

L

I

L

M

E

K

L

M

L

Y

K

S

E

V

H

H

G

G

F

I

K

K

E

D

V

F

M

I

V

I

N

C

V

C

S

G

H

Y

L

K

A

G

Q

Y

E

V

I

I

E

K

S

E

Y

Y

F

F

R

A

D

N

G

Y

Q

F

R

L

F

H

G

M

V

S

E

D

V

V

A

T

Y

F

S

H

F

M

-

A

E

E

G

N

R

R

I

M

E

E

D

V

G

H

Q

H

L

K

-

R

-

V

-

E

-

P

-

W

-

N

-

V

-

T

-

L

-

V

-

D

I

T

G

G

E

D

A

S

L
x
S

G

M

S

T

A

G

G

G

G
x
R

M

L

K

K

R

R

I

V

Q

A

D

E

F

Y

S

V

P

K

F

-

F

D

D

D

D

E

T

A

F

F

V

L

V

F

L

T

C

Y

G
|
G

D
|
D

A

G

L

V

I

A

D

D

L

L

D

D

L

I

T

K

E

A

A

T

V

I

R

D

W

F

H

H

K

K

E

A

K

H

G

G

A

K

L

K

A

A

T

T

I

L

V

T

M

A

K

T

T

F

V

P

P

P

R

-

E

-

V

-

S

G

S

R

Y

F

G

G

V

A

V

L

V

D

T

I

D

Q

S

A

D

-

G

G

R

Q

I

V

Q

R

A

S

F

F

Q

Q

E

E

K

K

P

P

S

K

V

G

E

D

E

G

A

A

L

M

S

-

T

-

I

I

N

N

T

G

G

G

I

F

Y

F

I

V

F

L

E

N

P

P

E

S

I

V

L

I

N

D

Y

L

I

I

K23 (= K23) binding
S104 (≠ L92) binding
R109 (≠ G97) binding
G128 (= G117) binding
D129 (= D118) binding

Sites not aligning to the query:

234 binding

Query Sequence

>Synpcc7942_1973 FitnessBrowser__SynE:Synpcc7942_1973
MKAMILAAGKGTRVRPITYTIPKPMIPILQKPVMEFLLELLKEHGFKEVMVNVSHLAQEI
ESYFRDGQRFGVEVAYSFEGRIEDGQLIGEALGSAGGMKRIQDFSPFFDDTFVVLCGDAL
IDLDLTEAVRWHKEKGALATIVMKTVPREEVSSYGVVVTDSDGRIQAFQEKPSVEEALST
TINTGIYIFEPEILNYIPSGQEYDIGGDLFPKLVETGAPFYGISMDFQWIDIGKVPDYWQ
AIRSVLNGEVRNVAIPGKQVRPGVYTGLNVAIDWDSVAIEGPVYIGGMTRIDEGVRIVGP
AMIGPNCHICKGATIENSVIFEYSRLGSGVRLVDKLVFGRYCVDRNGNAIDVQAAALDWL
ITDARRAEPVQPSAEHQAIADLLGSNVLP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory