SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Synpcc7942_2043 FitnessBrowser__SynE:Synpcc7942_2043 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q9WZC3 S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; EC 4.1.1.50 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
43% identity, 72% coverage: 17:116/139 of query aligns to 4:103/130 of Q9WZC3

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Q9WZC3

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S55 (≠ T68) mutation to A: Cleaves more rapidly than the wild-type.
S63 (= S76) mutation to A: Loss of processing.
H68 (= H81) mutation to A: Cleaves much more slowly than the wild-type, but the addition of hydroxylamine which is known to cleave ester bonds leads to the cleavage of this mutant.
C83 (= C96) mutation to A: Cleaves more rapidly than the wild-type.

Q57763 S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; EC 4.1.1.50 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
52% identity, 62% coverage: 17:102/139 of query aligns to 5:90/124 of Q57763

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S64 (= S76) modified: Pyruvic acid (Ser); by autocatalysis

O34426 S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; EC 4.1.1.50 from Bacillus subtilis (strain 168) (see paper)
41% identity, 72% coverage: 17:116/139 of query aligns to 4:102/126 of O34426

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O34426

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S63 (= S76) mutation to A: Loss of function.

Q9UWY8 S-adenosylmethionine decarboxylase proenzyme; AdoMetDC; SAMDC; EC 4.1.1.50 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 78% coverage: 17:125/139 of query aligns to 13:120/124 of Q9UWY8

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S71 (= S76) modified: Pyruvic acid (Ser); by autocatalysis

Q9UWU1 Arginine decarboxylase proenzyme; ADC; ArgDC; Pyruvoyl-dependent arginine decarboxylase; EC 4.1.1.19 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 75% coverage: 17:120/139 of query aligns to 21:126/134 of Q9UWU1

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S82 (= S76) modified: Pyruvic acid (Ser); by autocatalysis

2iiiA Crystal structure of the adenosylmethionine decarboxylase (aq_254) from aquifex aeolicus vf5
37% identity, 70% coverage: 14:110/139 of query aligns to 1:96/120 of 2iiiA

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active site: T55 (= T68), S62 (= S76), H67 (= H81), C82 (= C96)
binding calcium ion: E31 (≠ R44), K41 (≠ L54), S44 (≠ Q57)
binding magnesium ion: D18 (≠ E31), D21 (≠ N34), D25 (≠ Y38)

3iwdB T. Maritima adometdc complex with 5'-deoxy-5'-dimethyl thioadenosine (see paper)
32% identity, 42% coverage: 17:75/139 of query aligns to 3:61/61 of 3iwdB

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3iwdB

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binding 5'-deoxy-5'-(dimethyl-lambda~4~-sulfanyl)adenosine: F48 (= F62), Y51 (≠ Q65), G52 (= G66)

3iwcB T. Maritima adometdc complex with s-adenosylmethionine methyl ester (see paper)
32% identity, 42% coverage: 17:75/139 of query aligns to 3:61/61 of 3iwcB

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3iwcB

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binding s-adenosylmethionine methyl ester: F48 (= F62), L49 (≠ E63), Y51 (≠ Q65), G52 (= G66), V53 (= V67), S54 (≠ T68), I59 (≠ L73), S60 (≠ A74), E61 (= E75)

Query Sequence

>Synpcc7942_2043 FitnessBrowser__SynE:Synpcc7942_2043
MTVSLNPLLADPPALGVGRHCILEIYGCAGELLNDAAYVDRSIREAAIAAGATLLNQVCH
EFEPQGVTALALLAESHISIHTWPENGYAAVDVFTCGDHTQPEVACHHLIQAFKAEHHSL
HSIVRRPPAAIREHERVPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory