SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_008197813.1 NCBI__GCF_000166275.1:WP_008197813.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00348 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; L-3-hydroxyacyl CoA dehydrogenase; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Sus scrofa (Pig) (see paper)
31% identity, 38% coverage: 5:307/800 of query aligns to 27:312/314 of P00348

query
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P00348

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GGGLMG 34:39 (≠ GSGVMG 12:17) binding NAD(+)
D57 (= D35) binding NAD(+)
E122 (= E110) binding NAD(+)
K127 (= K115) binding NAD(+)
S149 (= S137) binding NAD(+)

1f17A L-3-hydroxyacyl-coa dehydrogenase complexed with nadh (see paper)
31% identity, 38% coverage: 2:307/800 of query aligns to 1:289/293 of 1f17A

query
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1f17A

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active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G14), L14 (≠ V15), M15 (= M16), D34 (= D35), Q35 (≠ E45), A96 (≠ V107), I97 (≠ V108), E99 (= E110), K104 (= K115), N124 (= N135), S126 (= S137), H147 (= H160)

1f12A L-3-hydroxyacyl-coa dehydrogenase complexed with 3-hydroxybutyryl-coa (see paper)
31% identity, 38% coverage: 2:307/800 of query aligns to 1:289/293 of 1f12A

query
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1f12A

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active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
binding 3-hydroxybutanoyl-coenzyme a: K57 (≠ S68), S126 (= S137), H147 (= H160), F149 (= F162), N150 (= N163), M155 (≠ L168), N197 (= N211), L200 (≠ G214), P232 (≠ K247), M233 (≠ S248), L238 (≠ T253)

1f0yA L-3-hydroxyacyl-coa dehydrogenase complexed with acetoacetyl-coa and NAD+ (see paper)
31% identity, 38% coverage: 2:307/800 of query aligns to 1:289/291 of 1f0yA

query
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1f0yA

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active site: S126 (= S137), H147 (= H160), E159 (= E172), N197 (= N211)
binding acetoacetyl-coenzyme a: S50 (≠ R60), K57 (≠ S68), S126 (= S137), H147 (= H160), F149 (= F162), N150 (= N163), P151 (= P164), P153 (≠ R166), V154 (≠ Y167), N197 (= N211), P232 (≠ K247), M233 (≠ S248), L238 (≠ T253)
binding nicotinamide-adenine-dinucleotide: G13 (= G14), L14 (≠ V15), M15 (= M16), D34 (= D35), Q35 (≠ E45), A96 (≠ V107), I97 (≠ V108), E99 (= E110), K104 (= K115), N124 (= N135), S126 (= S137), H147 (= H160), N150 (= N163), V242 (= V257), T246 (= T261), K282 (= K300)

Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; HCDH; Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; Short-chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35 from Homo sapiens (Human) (see 7 papers)
31% identity, 38% coverage: 2:307/800 of query aligns to 24:312/314 of Q16836

query
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Q16836

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GGGLMG 34:39 (≠ GSGVMG 12:17) binding NAD(+)
A40 (≠ S18) to T: in HADH deficiency; dbSNP:rs137853101
D57 (= D35) binding NAD(+); to E: in HADH deficiency; dbSNP:rs137853102; to G: found in a patient with Reye-like syndrome; does not affect 3-hydroxyacyl-CoA dehydrogenase activity; increases KM value for NADH; does not affect dimerization
S73 (≠ A56) binding CoA
K80 (≠ S64) binding CoA
L86 (= L70) to P: in dbSNP:rs4956145
E122 (= E110) binding NAD(+)
K127 (= K115) binding NAD(+); modified: N6-(2-hydroxyisobutyryl)lysine
S149 (= S137) binding CoA; binding NAD(+)
Q152 (≠ P140) to H: in dbSNP:rs1051519
N173 (= N163) binding NAD(+)
Y226 (≠ A217) to H: found in a patient with Reye-like syndrome; loss of 3-hydroxyacyl-CoA dehydrogenase activity. Does not affect dimerization; dbSNP:rs146036912
P258 (≠ T250) to L: in HHF4; loss of 3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137853103
K305 (= K300) binding NAD(+)

P9WNP7 3-hydroxybutyryl-CoA dehydrogenase; Beta-hydroxybutyryl-CoA dehydrogenase; BHBD; EC 1.1.1.157 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
28% identity, 38% coverage: 1:306/800 of query aligns to 1:284/286 of P9WNP7

query
sites
P9WNP7

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S122 (= S137) mutation to A: Loss of fatty acyl dehydrogenase activity.

4om8A Crystal structure of 5-formly-3-hydroxy-2-methylpyridine 4-carboxylic acid (fhmpc) 5-dehydrogenase, an NAD+ dependent dismutase. (see paper)
28% identity, 38% coverage: 5:306/800 of query aligns to 2:280/309 of 4om8A

query
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4om8A

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active site: S116 (= S137), H137 (= H160), E149 (= E172), N188 (= N211)
binding nicotinamide-adenine-dinucleotide: G11 (= G14), T12 (≠ V15), M13 (= M16), Y31 (≠ L34), D32 (= D35), V33 (≠ I36), N86 (≠ V107), V87 (= V108), P88 (≠ V109), E89 (= E110), K94 (= K115), D114 (≠ N135), H137 (= H160)

Q988C8 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylate 5-dehydrogenase; FHMPC dehydrogenase; EC 1.2.1.100 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
28% identity, 38% coverage: 5:306/800 of query aligns to 2:280/309 of Q988C8

query
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Q988C8

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L

-

R

S

E

A

A

M

V

H

D

T

L

V

V

E

E

K

R

M

G

G

V

L

I

G

D

V

P

S

E

E

D

V

L

D

D

K

T

M

C

T

V

G

S

T

W

V

G

I

I

G

G

R

Y

-

K

-

I

A

A

K

V

S

I

A

G

T

P

F

M

R

A

T

L

M

L

D

D

V

M

V

A

G

G

L

L

D

D

T

I

T

Y

V

K

N

S

V

V

A

S

N

S

N

F

L

L

Y

N

K

A

A

D

L

L

P

S

N

N

D

R

E

D

S

D

R

V

E

A

K

-

F

-

K

-

L

-

P

P

K

M

I

V

V

L

E

E

V

K

L

T

Y

S

E

A

N

S

K

K

W

-

F

F

G

G

D

I

K

K

T

S

G

G

H

E

G

G

Y

M

F

F

TM 12:13 (≠ VM 15:16) binding NAD(+)
D32 (= D35) binding NAD(+)
VPE 87:89 (≠ VVE 108:110) binding NAD(+)
K94 (= K115) binding NAD(+)
H137 (= H160) mutation to L: Loss of activity.
E149 (= E172) mutation to Q: Shows a different pH optimum depending on the cosubstrate.

1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
30% identity, 30% coverage: 100:336/800 of query aligns to 393:619/707 of 1wdmA

query
sites
1wdmA

D

N

Y

V

D

D

W

L

V

V

M

V

E

E

V

A

V
|
V

V

V

E
|
E

R

N

L

P

D

K

I

V

K

K

Q

Q

S

A

L

V

F

L

E

A

K

E

V

V

E

E

K

N

H

H

R

V

K

R

P

E

G

D

T

A

L

I

I

L

T

A

S

S

N
|
N

T

T

S
|
S

G

T

I

I

P

S

M

I

H

S

M

L

C

A

E

K

G

A

R

L

S

K

E

R

D

-

F

-

Q

P

E

E

N

N

F

F

A

V

G

G

T

M

H
|
H

F

F

N
|
N

P

P

P

V

R

H

Y

M

L
x
M

R

P

L

L

L

V

E
|
E

I

V

I

I

P

R

G

G

P

E

K

K

S

S

K

S

P

D

E

L

N

A

I

V

E

A

F

T

L

T

M

V

D

A

Y

Y

G

A

D

K

R

K

F

-

L

M

G

G

K

K

E

N

T

P

V

I

L

V

C

V

K

N

D

D

T

C

P

P

A

G

F

F

I

L

A

V

N
|
N

R

R

V

V

G

-

V

L

Y

F

A

P

I

Y

I

F

S

G

A

G

M

F

H

A

T

K

V

L

E

V

K

S

M

A

G

G

L

V

G

D

V

F

S

V

E

R

V

I

D

D

K

K

M

V

T

M

-

E

-

K

-

F

G

G

T

W

V
x
P

I

M

G

G

R

P

A

A

K

-

S

-

A

-

T

-

F

-

R

Y

T

L

M

M

D

D

V

V

G

G

L

I

D

D

T

T

T

G

V

H

N

H

V

G

A

R

N

D

N

V

L

M

Y

A

K

E

A

G

L

F

P

P

N

D

D

-

E

-

S

-

R

R

E

M

K

K

F

D

K

D

L

R

P

R

K

S

I

A

V

I

E

D

V

A

L

L

Y

Y

E

E

N

A

K

K

W

R

F

L

G

G

D

Q

K

K

T

N

G

G

H

K

G

G

Y

F

F

Y

K

A

M

Y

I

K

R

K

H

L

K

V

D

D

G

S

S

S

-

V

-

L

K

E

E

V

L

L

K

K

E

P

I

I

D

-

F

-

E

-

T

V

F

Y

E

E

Y

Q

K

R

D

D

V

V

E

T

K

D

P

E

N

D

I

I

active site: S430 (= S137), H451 (= H160), E463 (= E172), N501 (= N211)
binding acetyl coenzyme *a: M459 (≠ L168), N501 (= N211), P534 (≠ V242)
binding nicotinamide-adenine-dinucleotide: V401 (= V108), E403 (= E110), N428 (= N135), S430 (= S137), N454 (= N163)

Sites not aligning to the query:

active site: 69, 89, 93, 117, 120, 139, 140, 147, 148
binding acetyl coenzyme *a: 142, 297, 652, 658
binding nicotinamide-adenine-dinucleotide: 321, 322, 324, 325, 344

1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
30% identity, 29% coverage: 100:327/800 of query aligns to 393:610/715 of 1wdlA

query
sites
1wdlA

D

N

Y

V

D

D

W

L

V

V

M

V

E

E

V
x
A

V
|
V

V

V

E
|
E

R

N

L

P

D

K

I

V

K

K

Q

Q

S

A

L

V

F

L

E

A

K

E

V

V

E

E

K

N

H

H

R

V

K

R

P

E

G

D

T

A

L

I

I

L

T

A

S

S

N
|
N

T
|
T

S
|
S

G

T

I

I

P

S

M

I

H

S

M

L

C

A

E

K

G

A

R

L

S

K

E

R

D

-

F

-

Q

P

E

E

N

N

F

F

A

V

G

G

T

M

H
|
H

F

F

N

N

P

P

P

V

R

H

Y

M

L

M

R

P

L

L

L

V

E
|
E

I

V

I

I

P

R

G

G

P

E

K

K

S

S

K

S

P

D

E

L

N

A

I

V

E

A

F

T

L

T

M

V

D

A

Y

Y

G

A

D

K

R

K

F

-

L

M

G

G

K

K

E

N

T

P

V

I

L

V

C

V

K

N

D

D

T

C

P

P

A

G

F

F

I

L

A

V

N
|
N

R

R

V

V

G

-

V

L

Y

F

A

P

I

Y

I

F

S

G

A

G

M

F

H

A

T

K

V

L

E

V

K

S

M

A

G

G

L

V

G

D

V

F

S

V

E

R

V

I

D

D

K

K

M

V

T

M

-

E

-

K

-

F

G

G

T

W

V

P

I

M

G

G

R

P

A

A

K

-

S

-

A

-

T

-

F

-

R

Y

T

L

M

M

D

D

V

V

G

G

L

I

D

D

T

T

T

G

V

H

N

H

V

G

A

R

N

D

N

V

L

M

Y

A

K

E

A

G

L

F

P

P

N

D

D

-

E

-

S

-

R

R

E

M

K

K

F

D

K

D

L

R

P

R

K

S

I

A

V

I

E

D

V

A

L

L

Y

Y

E

E

N

A

K

K

W

R

F

L

G

G

D

Q

K

K

T

N

G

G

H

K

G

G

Y

F

F

Y

K

A

M

Y

I

E

R

A

H

D

K

K

D

K

G

G

S

-

K

K

E

Q

L

K

K

K

E

L

I

V

D

D

F

S

E

S

T

V

F

L

E

E

active site: S430 (= S137), H451 (= H160), E463 (= E172), N501 (= N211)
binding nicotinamide-adenine-dinucleotide: A400 (≠ V107), V401 (= V108), E403 (= E110), N428 (= N135), T429 (= T136), S430 (= S137)

Sites not aligning to the query:

active site: 69, 89, 93, 117, 120, 139, 140, 147, 148
binding nicotinamide-adenine-dinucleotide: 322, 324, 325, 344, 345

P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
30% identity, 29% coverage: 100:327/800 of query aligns to 393:610/715 of P28793

query
sites
P28793

D

N

Y

V

D

D

W

L

V

V

M

V

E

E

V

A

V
|
V

E
|
E

R

N

L

P

D

K

I

V

K
|
K

Q

Q

S

A

L

V

F

L

E

A

K

E

V

V

E

E

K

N

H

H

R

V

K

R

P

E

G

D

T

A

L

I

I

L

T

A

S

S

N

N

T

T

S
|
S

G

T

I

I

P

S

M

I

H

S

M

L

C

A

E

K

G

A

R

L

S

K

E

R

D

-

F

-

Q

P

E

E

N

N

F

F

A

V

G

G

T

M

H

H

F

F

N
|
N

P

P

P

V

R

H

Y

M

L

M

R

P

L

L

L

V

E

E

I

V

I

I

P

R

G

G

P

E

K

K

S

S

K

S

P

D

E

L

N

A

I

V

E

A

F

T

L

T

M

V

D

A

Y

Y

G

A

D

K

R

K

F

-

L

M

G

G

K

K

E

N

T

P

V

I

L

V

C

V

K

N

D

D

T

C

P

P

A

G

F

F

I

L

A

V

N
|
N

R

R

V

V

G

-

V

L

Y

F

A

P

I

Y

I

F

S

G

A

G

M

F

H

A

T

K

V

L

E

V

K

S

M

A

G

G

L

V

G

D

V

F

S

V

E

R

V

I

D

D

K

K

M

V

T

M

-

E

-

K

-

F

G

G

T

W

V

P

I

M

G

G

R

P

A

A

K

-

S

-

A

-

T

-

F

-

R

Y

T

L

M

M

D

D

V

V

G

G

L

I

D

D

T

T

T

G

V

H

N

H

V

G

A

R

N

D

N

V

L

M

Y

A

K

E

A

G

L

F

P

P

N

D

D

-

E

-

S

-

R

R

E

M

K

K

F

D

K

D

L

R

P

R

K

S

I

A

V

I

E

D

V

A

L

L

Y

Y

E

E

N

A

K

K

W

R

F

L

G

G

D

Q

K

K

T

N

G

G

H

K

G

G

Y

F

F

Y

K

A

M

Y

I

E

R

A

H

D

K

K

D

K

G

G

S

-

K

K

E

Q

L

K

K

K

E

L

I

V

D

D

F

S

E

S

T

V

F

L

E

E

VVE 401:403 (= VVE 108:110) binding NAD(+)
K408 (= K115) binding NAD(+)
S430 (= S137) binding NAD(+)
N454 (= N163) binding NAD(+)
N501 (= N211) binding substrate

Sites not aligning to the query:

297 binding substrate
325 binding NAD(+)
344 binding NAD(+)
660 binding substrate

6aa8E Crystal structure of (s)-3-hydroxybutyryl-coenzymea dehydrogenase from clostridium acetobutylicum complexed with NAD+ (see paper)
27% identity, 38% coverage: 6:306/800 of query aligns to 1:277/281 of 6aa8E

query
sites
6aa8E

K

K

N

K

V

V

A

C

I

V

L
x
I

G

G

S

A

G
|
G

V
x
T

M
|
M

G

G

S

S

R

G

I

I

A

A

C

Q

H

A

F

F

A

A

N

A

I

K

G

G

V

F

Q

E

V

V

L

V

L

L

L
x
R

D
|
D

I
|
I

V

-

P

-

F

-

E

-

L

-

T

K

E

D

Q

E

E

F

Q

V

K

D

K

R

G

G

L

L

T

D

K

-

E

-

D

-

P

-

A

-

V

-

R

-

N

-

R

-

L

F

V

I

N

N

S

K

A

N

L

L

Q

S

S

K

T

L

L

V

K

K

S

K

N

G

P

-

S

-

A

K

I

I

Y

E

D

E

K

A

A

T

F

K

A

V

D

E

R

I

I

L

Q

T

T

R

G

I

N

S

F

G

D

T

D

V

D

D

L

L

H

N

K

M

I

A

K

A

D

D

Y

C

D

D

W

L

V

V

M

I

E

E

V
x
A

V

V

E
|
E

R

R

L

M

D

D

I

I

K
|
K

Q

K

S

Q

L

I

F

F

E

A

K

D

V

L

E

D

K

N

H

I

R

C

K

K

P

P

G

E

T

T

L

I

I

L

T

A

S

S

N
|
N

T

T

S
|
S

G

S

I

L

P

S

M

I

H

T

M

E

M

V

C

A

E

S

G

A

R

T

S

K

E

R

D

-

F

-

Q

P

E

D

N

K

F

V

A

I

G

G

T

M

H
|
H

F

F

N

N

P

P

P

A

R

P

Y

V

L

M

R

K

L

L

L

V

E
|
E

I

V

I

I

P

R

G

G

P

I

K

A

S

T

K

S

P

Q

E

E

N

T

I

F

E

D

F

A

L

V

M

K

D

E

Y

T

G

S

D

I

R

A

F

-

L

I

G

G

K

K

E

D

T

P

V

V

L

E

C

V

K

A

D

E

T

A

P

P

A

G

F

F

I

V

A

V

N
|
N

R

R

V

I

G

L

V

I

Y

P

A

M

I

I

I

N

S

E

A

A

M

V

H

G

T

I

V

L

E

A

K

E

M

G

G

I

L

A

G

S

V

V

S

E

E

D

V

I

D

D

K

K

M

A

T

M

G

-

T

K

V

L

I

G

G

A

R

N

A

H

K

P

S

M

A

G

T

P

F

L

R

E

T

L

M

G

D

D

V

F

V

I

G

G

L

L

D

D

T

I

T

C

V

L

N

A

V

I

A

M

N

D

N

V

L

L

Y

Y

K

S

A

E

L

T

P

G

N

D

D

S

E

K

S

Y

R

R

E

P

K

H

F

T

K

L

L

L

P

K

K

K

I

Y

V

V

E

R

V

A

L

-

Y

-

E

-

N

-

K

G

W

W

F

L

G

G

D

R

K

K

T

S

G

G

H

K

G

G

Y

F

F

Y

active site: S116 (= S137), H137 (= H160), E149 (= E172), N187 (= N211)
binding nicotinamide-adenine-dinucleotide: I6 (≠ L11), G9 (= G14), T10 (≠ V15), M11 (= M16), R29 (≠ L34), D30 (= D35), I31 (= I36), A86 (≠ V107), A87 (≠ V108), E89 (= E110), K94 (= K115), N114 (= N135), S116 (= S137)

6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
28% identity, 29% coverage: 84:316/800 of query aligns to 370:597/719 of 6tnmA

query
sites
6tnmA

D

D

R

G

I

L

Q

K

T

L

G

A

N

G

F

V

D

I

D

S

D

T

L

I

H

H

K

P

I

T

K

L

D

D

Y

Y

-

A

-

G

-

F

-

D

-

R

-

V

D

D

W

I

V

V

M

V

E

E

V

A

V
|
V

E

E

R

N

L

P

D

K

I
x
V

K

K

Q

K

S

A

L

V

F

L

E

A

K

E

V

T

E

E

K

Q

H

K

R

V

K

R

P

Q

G

D

T

T

L

V

I

L

T

A

S

S

N

N

T

T

S
|
S

G

T

I

I

P

P

M

I

H

S

M

E

M

L

C

A

E

N

G

A

R

L

S

E

E

R

D

-

F

-

Q

P

E

E

N

N

F

F

A

C

G

G

T

M

H
|
H

F

F

N

N

P

P

P

V

R

H

Y

R

L

M

R

P

L

L

L

V

E
|
E

I

I

P

R

G

G

P

E

K

K

S

S

K

S

P

D

E

E

N

T

I

I

E

A

F

K

L

V

M

V

D

A

Y

W

G

A

D

S

R

K

F

-

L

M

G

G

K

K

E

T

T

P

V

I

L

V

C

V

K

N

D

D

T

C

P

P

A

G

F

F

I

F

A

V

N
|
N

R

R

V

V

G

-

V

L

Y

F

A

P

I

Y

I

F

S

A

A

G

M

F

H

S

T

Q

V

L

E

L

K

R

M

D

G

G

L

A

G

D

V

F

S

R

E

K

V

I

D

D

K

K

M

V

T

M

G

E

T

K

V

Q

I

F

G

G

R

W

A

P

K

M

S

G

A

P

T

A

F

Y

R

-

T

L

M

L

D

D

V

V

G

G

L

I

D

D

T

T

T

A

V

H

N

H

V

A

A

Q

N

A

N

V

L

M

Y

A

K

A

A

G

L

F

P

P

N

Q

D

-

E

-

S

-

R

R

E

M

K

Q

F

K

K

D

L

Y

P

R

K

D

I

A

V

I

E

D

V

A

L

L

Y

F

E

D

N

A

K

N

W

R

F

F

G

G

D

Q

K
|
K

T

N

G

G

H

L

G

G

Y

F

F

W

K

-

M

-

I

-

R

R

H

Y

K

K

D

E

G

D

S

S

K

K

active site: S429 (= S137), H450 (= H160), E462 (= E172), N500 (= N211)
binding adenosine-5'-triphosphate: V400 (= V108), V401 (= V109), V406 (≠ I114), K584 (= K300)

Sites not aligning to the query:

active site: 68, 73, 116, 119, 138, 139, 147, 271
binding adenosine-5'-triphosphate: 343, 344

P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
28% identity, 29% coverage: 84:316/800 of query aligns to 370:597/729 of P21177

query
sites
P21177

D

D

R

G

I

L

Q

K

T

L

G

A

N

G

F

V

D

I

D

S

D

T

L

I

H

H

K

P

I

T

K

L

D

D

Y

Y

-

A

-

G

-

F

-

D

-

R

-

V

D

D

W

I

V

V

M

V

E

E

V

A

V

V

E

E

R

N

L

P

D

K

I

V

K

K

Q

K

S

A

L

V

F

L

E

A

K

E

V

T

E

E

K

Q

H

K

R

V

K

R

P

Q

G

D

T

T

L

V

I

L

T

A

S

S

N

N

T

T

S

S

G

T

I

I

P

P

M

I

H

S

M

E

M

L

C

A

E

N

G

A

R

L

S

E

E

R

D

-

F

-

Q

P

E

E

N

N

F

F

A

C

G

G

T

M

H
|
H

F

F

N

N

P

P

P

V

R

H

Y

R

L

M

R

P

L

L

L

V

E

E

I

I

P

R

G

G

P

E

K

K

S

S

K

S

P

D

E

E

N

T

I

I

E

A

F

K

L

V

M

V

D

A

Y

W

G

A

D

S

R

K

F

-

L

M

G

G

K

K

E

T

T

P

V

I

L

V

C

V

K

N

D

D

T

C

P

P

A

G

F

F

I

F

A

V

N

N

R

R

V

V

G

-

V

L

Y

F

A

P

I

Y

I

F

S

A

A

G

M

F

H

S

T

Q

V

L

E

L

K

R

M

D

G

G

L

A

G

D

V

F

S

R

E

K

V

I

D

D

K

K

M

V

T

M

G

E

T

K

V

Q

I

F

G

G

R

W

A

P

K

M

S

G

A

P

T

A

F

Y

R

-

T

L

M

L

D

D

V

V

G

G

L

I

D

D

T

T

T

A

V

H

N

H

V

A

A

Q

N

A

N

V

L

M

Y

A

K

A

A

G

L

F

P

P

N

Q

D

-

E

-

S

-

R

R

E

M

K

Q

F

K

K

D

L

Y

P

R

K

D

I

A

V

I

E

D

V

A

L

L

Y

F

E

D

N

A

K

N

W

R

F

F

G

G

D

Q

K

K

T

N

G

G

H

L

G

G

Y

F

F

W

K

-

M

-

I

-

R

R

H

Y

K

K

D

E

G

D

S

S

K

K

H450 (= H160) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.

Sites not aligning to the query:

116 G→F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
322 G→A: 10-fold increase in KM for NADH.

6zicAAA structure of rat peroxisomal multifunctional enzyme type-1 (rpmfe1) complexed with 3s-hydroxybutanoyl-coa and nadh'
25% identity, 53% coverage: 3:422/800 of query aligns to 299:682/723 of 6zicAAA

query
sites
6zicAAA

R

Q

T

P

I

V

K

S

N

S

V

V

A

G

I

V

L

L

G
|
G

S

L

G
|
G

V
x
T

M
|
M

G

G

S

R

R

G

I

I

A

A

C

I

H

S

F

F

A

A

N

R

I

V

G

G

V

I

Q

S

V

V

L

V

L

A

L

V

D
x
E

I
x
S

V

D

P

P

F

K

E
x
Q

L

L

T

D

E

A

Q

A

E

K

Q

K

K

I

G

T

L

F

T

T

K

L

E

E

D

K

P

E

A

A

V

S

R

R

N

A

R

H

L

Q

V

-

N

N

S

G

A

S

L

A

Q

K

S

P

T

K

L

L

K

R

S

-

N

-

P

-

S

-

A

-

I

-

Y

-

D

-

K

-

A

-

F

-

A

-

D

-

R

-

I

-

Q

-

T

-

G

-

N

-

F

F

D

S

L

T

H

K

K

E

I

L

K

S

D

T

Y

V

D

D

W

L

V

V

M

V

E

E

V
x
A

V
|
V

V
x
F

E
|
E

R

D

L

M

D

N

I
x
L

K
|
K

Q

K

S

K

L

V

F

F

E

A

K

E

V

L

E

S

K

A

H

L

R

C

K

K

P

P

G

G

T

A

L

F

I

L

T

C

S

T

N
|
N

T

T

S
|
S

G

A

I

L

P

N

M

V

H

D

M

D

M

I

C

A

E

S

G

-

R

-

S

S

E

T

D

D

F

R

Q

P

E

Q

N

L

F

V

A

I

G

G

T

T

H
|
H

F

F

N

S

P

P

P

A

R

H

Y

V

L

M

R

R

L

L

E
|
E

I

V

I

I

P

P

G

S

P

R

K

Y

S

S

K

S

P

P

E

T

N

T

I

I

E

A

F

T

L

V

M

M

D

S

Y

L

G

S

D

K

R

K

F

-

L

I

G

G

K

K

E

I

T

G

V

V

L

V

C

V

K

G

D

N

T

C

P

Y

A

G

F

F

I

V

A

G

N
|
N

R

R

V

M

G

L

V

A

Y

P

A

Y

I

Y

I

N

S

Q

A

G

M

F

H

F

T

L

V

L

E

E

K

E

M

-

G

G

L

S

G

K

V

P

S

E

E

D

V

V

D

D

K

G

M

V

T

L

G

E

T

E

V

F

I

-

G

-

R

G

A

F

K

K

S

M

A

G

T

P

F

F

R

R

T

V

M

S

D

D

V

L

V

A

G

G

L

L

D

D

T

V

T

G

V

W

N

K

V

I

A

R

N

K

N

G

-

Q

-

G

-

L

L

T

Y

G

K

P

A

S

L

L

P

P

N

P

D

G

-

T

-

P

-

V

E

R

S

K

R

R

E

G

K

N

F

S

K

R

L

Y

P

S

K

P

I

L

V

G

E

D

V

M

L

L

Y

C

E

E

N

A

K

G

W

R

F

F

G

G

D

Q

K

K

T

T

G

G

H

K

G

G

Y

W

F

Y

K

Q

M

Y

I

D

R

K

H

-

K

P

D

L

G

G

S

R

K

I

E

H

L

K

K

P

E

D

I

P

D

W

F

L

E

S

T

T

F

F

-

L

-

S

E

Q

Y

Y

K

R

D

E

V

V

E

H

K

-

P

-

N

H

I

I

K

E

A

Q

L

R

Q

T

A

I

S

S

K

K

E

E

I

-

E

E

D

I

L

L

K

E

K

R

R

C

I

L

K

Y

F

S

L

L

V

I

N

N

F

-

D

-

K

-

A

-

G

-

E

-

F

-

Y

-

R

-

T

E

T

A

F

F

Y

R

D

I

L

L

F

E

K

E

Y

G

C

M

S

A

N

A

R

R

I

-

P

P

E

E

I

H

S

I

D

D

E

V

L

I

Y

Y

R

-

I

-

D

-

Q

-

A

-

V

-

C

L

A

H

G

G

F

Y

G

G

W

W

E

P

L

R

-

H

-

K

-

G

G

G

P

P

F

M

E

F

T

Y

W

A

D

A

V

S

L

V

G

G

V

L

K

P

E

T

T

V

V

L

E

E

K

K

M

L

E

Q

active site: S413 (= S137), H434 (= H160), E446 (= E172), N484 (= N211)
binding 1,4-dihydronicotinamide adenine dinucleotide: G308 (= G12), G310 (= G14), T311 (≠ V15), M312 (= M16), E331 (≠ D35), S332 (≠ I36), Q336 (≠ E40), A383 (≠ V107), V384 (= V108), F385 (≠ V109), E386 (= E110), L390 (≠ I114), K391 (= K115), N411 (= N135), S413 (= S137), H434 (= H160)

Sites not aligning to the query:

active site: 66, 71, 81, 105, 108, 127, 128, 136, 254
binding 3-hydroxybutanoyl-coenzyme a: 25, 26, 28, 66, 67, 68, 104, 105, 108, 128, 161

6iunB Crystal structure of enoyl-coa hydratase (ech) from ralstonia eutropha h16 in complex with NAD
25% identity, 54% coverage: 3:431/800 of query aligns to 289:674/692 of 6iunB

query
sites
6iunB

R

R

T

K

I

I

K

E

N

K

V

V

A

A

I

V

L

I

G

G

S

A

G
|
G

V
x
T

M
|
M

G

G

S

G

R

G

I

I

A

S

C

M