Align Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 (characterized)
to candidate WP_011321519.1 AVA_RS24665 glutamate--tRNA ligase
Query= SwissProt::Q8DLI5 (485 letters) >NCBI__GCF_000204075.1:WP_011321519.1 Length = 481 Score = 627 bits (1616), Expect = 0.0 Identities = 307/478 (64%), Positives = 366/478 (76%), Gaps = 1/478 (0%) Query: 1 MTVRVRLAPSPTGNLHIGTARTAVFNWLYARHRGGKFILRIEDTDRERSRPEYTENILEG 60 MTVRVR+APSPTGNLHIGTARTAVFNWL+ARH GG FILRIEDTD ERSRPEYTENI+ G Sbjct: 1 MTVRVRIAPSPTGNLHIGTARTAVFNWLFARHHGGTFILRIEDTDLERSRPEYTENIMTG 60 Query: 61 LQWLGLTWDEGPYFQSDRLDLYRQAIQTLLDKGLAYYCYCTPEELEALRAEQKAKGQAPR 120 L+WLGL WDEGP+FQS RLDLY++A++ LLD+GLAY CY T EELEALR QKAKG+APR Sbjct: 61 LRWLGLNWDEGPFFQSQRLDLYQKAVKQLLDQGLAYRCYTTSEELEALREAQKAKGEAPR 120 Query: 121 YDNRHRHLTPEEQAAFEAAGRTPVIRFKIEDDRQIEWQDLVRGRVSWQGADLGGDMVIAR 180 YDNRHRHLTPE++A F+A GR+ VIRFKI+D+R+I W DLVRG++SW+G+DLGGDMVIAR Sbjct: 121 YDNRHRHLTPEQEAEFKAQGRSFVIRFKIDDEREIVWNDLVRGKMSWRGSDLGGDMVIAR 180 Query: 181 AAPRGEIGYPLYNLVVVVDDIAMGITDVIRGEDHIGNTPKQILLYEALGATPPNFAHTPL 240 A+ + G PLYN VVV+DDI M I+ VIRGEDHI NT KQILLYEA GA P FAHTPL Sbjct: 181 AS-ENDTGQPLYNFVVVIDDIDMQISHVIRGEDHIANTAKQILLYEAFGAKIPEFAHTPL 239 Query: 241 ILNSTGQKLSKRDGVTSISDFRAMGYLAPALANYMTLLGWSPPEGVGELFTLDLAAKHFS 300 ILN G+KLSKRDGVTSISDF+ MG+ + L NYMTLLGWSPP+ E+FTL+ AAK F+ Sbjct: 240 ILNMEGRKLSKRDGVTSISDFQQMGFTSEGLVNYMTLLGWSPPDSTQEIFTLEAAAKEFT 299 Query: 301 FERINKAGARFDWDKLNWLNRQYIQQLEPEEFLAELIPLWQGAGYAFDEERDRPWLFDLA 360 FER+NKAGA+FDW KL+WLN QYI ++ LIP W+ AGY+F RDRPWL L Sbjct: 300 FERVNKAGAKFDWAKLDWLNSQYIHNTPVDQLTDLLIPYWEAAGYSFAGGRDRPWLEQLV 359 Query: 361 QLLQPGLNTLREAIDQGAVFFIPSVTFDSEAMAQLGQPQSATILAYLLEHLPAEPALTVA 420 LL L L +A+D +FF +V E QL Q S +L ++ L A+ LT Sbjct: 360 GLLSASLTRLTDAVDMSKLFFSETVELSEEGSKQLQQEGSKAVLEAIIAALEAQTQLTEN 419 Query: 421 MGQQLIQQAAKAAGVKKGATMRTLRAALTGAVHGPDLMAAWQILHQRGWDEPRLAAAL 478 Q +I+Q KA VKKG MR+LR ALTG VHGPDL+ +W +L+Q G D+PRL+ A+ Sbjct: 420 AAQDIIKQVVKAQNVKKGLVMRSLRVALTGDVHGPDLIQSWLLLNQIGLDKPRLSQAI 477 Lambda K H 0.320 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 735 Number of extensions: 25 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 485 Length of database: 481 Length adjustment: 34 Effective length of query: 451 Effective length of database: 447 Effective search space: 201597 Effective search space used: 201597 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory