Annotation: FitnessBrowser__SynE:Synpcc7942_1501
Length: 546 amino acids
Source: SynE in FitnessBrowser
Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
---|---|---|---|---|---|---|---|---|---|
L-cysteine biosynthesis | serA | hi | D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95 (characterized, see rationale) | 45% | 100% | 437.2 | |||
L-cysteine biosynthesis | serA | hi | serA: phosphoglycerate dehydrogenase (EC 1.1.1.95) (TIGR01327) | 100% | 703.6 | ||||
L-serine biosynthesis | serA | hi | D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95 (characterized, see rationale) | 45% | 100% | 437.2 | |||
L-serine biosynthesis | serA | hi | serA: phosphoglycerate dehydrogenase (EC 1.1.1.95) (TIGR01327) | 100% | 703.6 | ||||
L-serine biosynthesis | serB | lo | fused D-3-phosphoglycerate dehydrogenase / phosphoserine phosphatase (EC 1.1.1.95; EC 3.1.3.3) (characterized) | 30% | 54% | 179.5 | glycerate dehydrogenase (EC 1.1.1.29) | 77% | 803.1 |
View Synpcc7942_1501 at FitnessBrowser
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
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Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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MIGRICGSSIPVARWLTMPKVLVSDPIDQVGLDILSQVAQVDVKTGLSPSELAQIIGEYD ALMLRSGTRVTAEVIEAGQKLRIIGRAGVGVDNVDVPAATRRGIVVVNSPEGNTIAAAEH TLAMMLSLSRHIPDANASTKSGGWDRKSFVGTEVYKKTLGVVGLGKIGSHVATVAKAMGM KLLAYDPFISAERAEQIGARLVELDILFQEADYITLHIPKTPETANLINAETLAKMKPTT RIINCARGGVINEQALADAIAAGKIGGAALDVYDQEPLQADSPLRALGKNLILTPHLGAS TTEAQVNVAVDVAEQIRDVLLGLPARSAVNIPGLYPDILEKLRPYLQLAETLGNLLSQVA GGRLEQLTVRLQGELAAQQSQPIVVAALKGLLTQALRERVNYVNAMIEAKERGIRIIETR DESVRDDYAGGSLQLVATGSNGEHRVMGALLGDGEIRITNVDEFPVNVPPSRYMLFTRHR DMPGIIGKIGSLLGSFNVNIASMQVGRRIVRGDAVMVLSLDDPLPEGILAEITKVAGISD AYTVVL
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory